Phosphopeptide enrichment with stable spatial coordination on a titanium dioxide coated glass slide

Susumu Imanishi, Kouvonen, Jan-Henrik Smått, Heikkilä, Emilia Peuhu, Mikhailov, Ritala, Lindén, Corthals, John Eriksson

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    3 Citations (Scopus)

    Abstract

    Recent advances in phosphoproteomics have established powerful tools to analyze phosphorylation events. However, their spatial localization is lost due to sample homogenization procedures prior to the analysis. Imaging mass spectrometry (IMS) has emerged as a method to visualize the spatial distribution of molecules in tissue samples, but its application is still limited to relatively abundant molecules. Due to low phosphorylation stoichiometry, direct detection and imaging of protein phosphorylation by MS has not been achieved yet. Therefore we have developed a novel phosphopeptide enrichment strategy as a potential tool for in situ affinity imaging MS (AIMS). A specific type of titanium dioxide (TiO2)-coated glass slides was designed and validated with casein tryptic digests for their ability to selectively retain phosphopeptides while maintaining their spatial coordination.
    Original languageUndefined/Unknown
    Pages (from-to)3661–3667
    JournalRapid Communications in Mass Spectrometry
    Volume23
    Issue number23
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

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