Oxidation of polysaccharides by galactose oxidase

Kirsti Parikka*, Ann Sofie Leppänen, Leena Pitkänen, Markku Reunanen, Stefan Willför, Maija Tenkanen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

74 Citations (Scopus)

Abstract

Galactose oxidase was used as a catalyst to oxidize selectively the C-6 hydroxyls of terminal galactose to carbonyl groups. The polysaccharides studied Included spruce galactoglucomannan, guar galactomannan, larch arabinogalactan, corn fiber arabinoxylan, and tamarind seed xyloglucan, with terminal galactose contents varying from 6% to 40%. A multienzyme system was used, with catalase and horseradish peroxidase to enhance the action of galactose oxidase. An analysis technique was developed for the quantification of the reactive aldehydes with GC-MS, utilizing NaBD4 reduction and acidic methanolysis. The best oxidation degrees of terminal galactosyl were obtained with xyloglucan (85% of galactose) and spruce galactoglucomannan (65% of galactose). The highest oxidation degree based on total carbohydrates was achieved with guar gum (28%), which had the highest galactose content. The oxidation resulted in changes in the physicochemical properties of the polysaccharide solutions, and the changes observed varied between the polysaccharides. The clearest change was in tamarind xyloglucan, which formed a gel after the oxidation. After the oxidation, larger particles were present in the solution of spruce galactoglucomannan, but changes in its rheological properties were not observed.

Original languageEnglish
Pages (from-to)262-271
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number1
DOIs
Publication statusPublished - 13 Jan 2010
MoE publication typeA1 Journal article-refereed

Keywords

  • Arabinogalactan
  • Arabinoxylan
  • Enzymatic oxidation
  • Galactoglucomannan
  • Galactomannan
  • Galactose oxidase
  • Xyloglucan

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