Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment

Alexander Leitner, Martin Sturm, Jan-Henrik Smått, Mikael Järn, Mika Lindén, Karl Mechtler, Wolfgang Lindner

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    Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.
    Original languageUndefined/Unknown
    Pages (from-to)51–7
    JournalAnalytica Chimica Acta
    Issue number1
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

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