On the relationship between the conserved 'black' and 'gray' structural clusters and intrinsic disorder in parvalbumins.

EI Deryusheva, Alexandre Denesyuk, Konstantin Denessiouk, VN Uversky, SE Permyakov, Permyakov EA. [Unknown]

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Recently we found two highly conserved structural motifs in the members of the EF-hand protein family, which provide a supporting scaffold for their Ca2+ binding loops. Each structural motif is formed by a cluster of three amino acids. These clusters were called 'black' cluster (cluster I) and 'gray' cluster (cluster II). In the present work, we studied the relationship between the location of the 'black' and 'gray' structural clusters in parvalbumins and the location of the amino acid sequence regions with a tendency for intrinsic disorder. This analysis revealed that in parvalbumins, the residues in the vicinity of the conserved structural clusters constitute parts of the conserved motifs enriched in the disorder-promoting residues. Therefore, the clusters found in parvalbumins are characterized not only by the presence of conserved amino acid residues, but also by the conserved distribution of the intrinsic disorder predisposition within their sequences, suggesting the presence of conserved structural dynamics in the apo-forms of parvalbumins, where the black cluster appears to have greater mobility than the gray cluster.

Original languageUndefined/Unknown
Pages (from-to)1055–1062
JournalInternational Journal of Biological Macromolecules
Issue numberA
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed

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