Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.
|Title of host publication||Ras Signalling|
|Editors||Lorenza Trabalzini, Saverio Francesco Retta|
|Publication status||Published - 2014|
|MoE publication type||A3 Part of a book or another research book|