Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

Camilo Guzman, Maja Solman, Daniel Abankwa

Research output: Chapter in Book/Conference proceedingChapterScientificpeer-review

3 Citations (Scopus)

Abstract

Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.
Original languageUndefined/Unknown
Title of host publicationRas Signalling
EditorsLorenza Trabalzini, Saverio Francesco Retta
PublisherHumana Press
Pages307–326
ISBN (Electronic)978-1-62703-791-4
ISBN (Print)978-1-62703-790-7
DOIs
Publication statusPublished - 2014
MoE publication typeA3 Part of a book or another research book

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