MYO10-filopodia support basement membranes at pre-invasive tumor boundaries

Emilia Peuhu, Guillaume Jacquemet, Colinda L G J Scheele, Aleksi Isomursu, Marie-Catherine Laisne, Leena M Koskinen, Ilkka Paatero, Kerstin Thol, Maria Georgiadou, Camilo Guzmán, Satu Koskinen, Asta Laiho, Laura L Elo, Pia Boström, Pauliina Hartiala, Jacco van Rheenen, Johanna Ivaska

Research output: Contribution to journalArticleScientificpeer-review

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Ductal carcinoma in situ (DCIS) is a pre-invasive stage of breast cancer. During invasion, the encapsulating DCIS basement membrane (BM) is compromised, and tumor cells invade the surrounding stroma. The mechanisms that regulate functional epithelial BMs in vivo are poorly understood. Myosin-X (MYO10) is a filopodia-inducing protein associated with metastasis and poor clinical outcome in invasive breast cancer (IBC). We identify elevated MYO10 expression in human DCIS and IBC, and this suggests links with disease progression. MYO10 promotes filopodia formation and cell invasion in vitro and cancer-cell dissemination from progressively invasive human DCIS xenografts. However, MYO10-depleted xenografts are more invasive. These lesions exhibit compromised BMs, poorly defined borders, and increased cancer-cell dispersal and EMT-marker-positive cells. In addition, cancer spheroids are dependent on MYO10-filopodia to generate a near-continuous extracellular matrix boundary. Thus, MYO10 is protective in early-stage breast cancer, correlating with tumor-limiting BMs, and pro-invasive at later stages, facilitating cancer-cell dissemination.

Original languageEnglish
Pages (from-to)2350-2364.e7
Number of pages24
JournalDevelopmental Cell
Issue number20
Publication statusPublished - 24 Oct 2022
MoE publication typeA1 Journal article-refereed


  • Humans
  • Female
  • Carcinoma, Intraductal, Noninfiltrating/metabolism
  • Pseudopodia/metabolism
  • Breast Neoplasms/pathology
  • Myosins/metabolism
  • Basement Membrane/metabolism
  • Carcinoma, Ductal, Breast/metabolism


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