Mussel-Inspired and Bioclickable Peptide Engineered Surface to Combat Thrombosis and Infection

Xiaohui Mou, Hongbo Zhang, Hua Qiu, Wentai Zhang, Ying Wang, Kaiqin Xiong, Nan Huang, Hélder A. Santos, Zhilu Yang

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)
123 Downloads (Pure)

Abstract

Thrombosis and infections are the two major complications associated with extracorporeal circuits and indwelling medical devices, leading to significant mortality in clinic. To address this issue, here, we report a biomimetic surface engineering strategy by the integration of mussel-inspired adhesive peptide, with bio-orthogonal click chemistry, to tailor the surface functionalities of tubing and catheters. Inspired by mussel adhesive foot protein, a bioclickable peptide mimic (DOPA) 4-azide-based structure is designed and grafted on an aminated tubing robustly based on catechol-amine chemistry. Then, the dibenzylcyclooctyne (DBCO) modified nitric oxide generating species of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelated copper ions and the DBCO-modified antimicrobial peptide (DBCO-AMP) are clicked onto the grafted surfaces via bio-orthogonal reaction. The combination of the robustly grafted AMP and Cu-DOTA endows the modified tubing with durable antimicrobial properties and ability in long-term catalytically generating NO from endogenous s-nitrosothiols to resist adhesion/activation of platelets, thus preventing the formation of thrombosis. Overall, this biomimetic surface engineering technology provides a promising solution for multicomponent surface functionalization and the surface bioengineering of biomedical devices with enhanced clinical performance.

Original languageEnglish
Article number9780879
JournalResearch
Volume2022
DOIs
Publication statusPublished - 14 Apr 2022
MoE publication typeA1 Journal article-refereed

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