MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes

M Bastiani, LB Liu, MM Hill, MP Jedrychowski, SJ Nixon, Lo HP, Daniel Abankwa, R Luetterforst, M Fernandez-Rojo, MR Breen, SP Gygi, J Vinten, PJ Walser, KN North, JF Hancock, PF Pilch, RG Parton

Research output: Contribution to journalArticleScientificpeer-review

193 Citations (Scopus)

Abstract

Polymerase I and transcript release factor (PTRF)/Cavin is a cytoplasmic protein whose expression is obligatory for caveola formation. Using biochemistry and fluorescence resonance energy transfer-based approaches, we now show that a family of related proteins, PTRF/Cavin-1, serum deprivation response (SDR)/Cavin-2, SDR-related gene product that binds to C kinase (SRBC)/Cavin-3, and muscle-restricted coiled-coil protein (MURC)/Cavin-4, forms a multiprotein complex that associates with caveolae. This complex can constitutively assemble in the cytosol and associate with caveolin at plasma membrane caveolae. Cavin-1, but not other cavins, can induce caveola formation in a heterologous system and is required for the recruitment of the cavin complex to caveolae. The tissue-restricted expression of cavins suggests that caveolae may perform tissue-specific functions regulated by the composition of the cavin complex. Cavin-4 is expressed predominantly in muscle, and its distribution is perturbed in human muscle disease associated with Caveolin-3 dysfunction, identifying Cavin-4 as a novel muscle disease candidate caveolar protein.
Original languageUndefined/Unknown
Pages (from-to)1259–1273
Number of pages15
JournalJournal of Cell Biology
Volume185
Issue number7
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

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