Multifunctional Cellulose Beads and Their Interaction with Gram Positive Bacteria

Leandro Blachechen, Pedro Fardim, Denise F. S. Petri

    Research output: Contribution to journalArticleScientificpeer-review

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    Abstract

    Cellulose beads with 3 mm of diameter and high circularity were obtained by dripping cellulose solutions (5, 6, and 7 wt 96) dissolved in NaOH7%/urea12%, into HCl 2 M coagulation bath. Carboxylic groups were generated on beads surface through NaClO/NaClO2/TEMPO oxidation method, achieving total charge density of similar to 0.77 mmol/g. Pristine (CB) and oxidized (OCB) beads were characterized by means of optical images analyses, scanning electron microscopy (SEM) and compression tests. Both types of beads, CB and OCB, were used as adsorbent for poly(4-vinyl-N-pentylpyridinium) bromide, QPVP-C5, a bactericidal agent. The adsorption of QPVP-C5 on CB and OCB was evaluated by means of FTIR-ATR, UV-vis, CHN elemental analyses, and X-ray photoelectron spectroscopy (XPS). The adsorbed amount of QPVP-C5 was remarkably higher on OCB than on CB due to ionic interactions. Desorption was less than 5%. The interaction between neat OCB or OCB coated and two different amounts of QPVP-C5 and Gram-positive bacteria Micrococcus luteus was assessed by changes in turbidimetry, SEM, and elemental analyses. Bacteria adsorbed on the surface of neat OCB and weakly QPVP-C5 coated OCB due to hydrogen bonding or ion-dipole interaction. Notorious bactericidal action was observed for OCB samples coated with large amount of QPVP-C5.
    Original languageUndefined/Unknown
    Pages (from-to)3440–3448
    Number of pages9
    JournalBiomacromolecules
    Volume15
    Issue number9
    DOIs
    Publication statusPublished - 2014
    MoE publication typeA1 Journal article-refereed

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