Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation

Otto K. Kari, Tatu Rojalin, Stefano Salmaso, Michela Barattin, Hanna Jarva, Seppo Meri, Marjo Yliperttula, Tapani Viitala*, Arto Urtti

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

When nanocarriers are administered into the blood circulation, a complex biomolecular layer known as the “protein corona” associates with their surface. Although the drivers of corona formation are not known, it is widely accepted that this layer mediates biological interactions of the nanocarrier with its surroundings. Label-free optical methods can be used to study protein corona formation without interfering with its dynamics. We demonstrate the proof-of-concept for a multi-parametric surface plasmon resonance (MP-SPR) technique in monitoring the formation of a protein corona on surface-immobilized liposomes subjected to flowing 100 % human serum. We observed the formation of formulation-dependent “hard” and “soft” coronas with distinct refractive indices, layer thicknesses, and surface mass densities. MP-SPR was also employed to determine the affinity (KD) of a complement system molecule (C3b) with cationic liposomes with and without polyethylene glycol. Tendency to create a thick corona correlated with a higher affinity of opsonin C3b for the surface. The label-free platform provides a fast and robust preclinical tool for tuning nanocarrier surface architecture and composition to control protein corona formation.

Original languageEnglish
Pages (from-to)228-240
Number of pages13
JournalDrug Delivery and Translational Research
Volume7
Issue number2
DOIs
Publication statusPublished - 1 Apr 2017
Externally publishedYes
MoE publication typeA1 Journal article-refereed

Keywords

  • Complement system
  • Liposome
  • Multi-parametric surface plasmon resonance (MP-SPR)
  • Opsonin
  • Protein corona
  • Soft corona

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