Modulation of virulence factors of Staphylococcus aureus by nanostructured surfaces

Paola San-Martin-Galindo*, Emil Rosqvist*, Stiina Tolvanen, Ilkka Miettinen, Kirsi Savijoki, Tuula A. Nyman, Adyary Fallarero, Jouko Peltonen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Investigating and understanding the response of microbes to various surfaces requires a versatile parametrisation of the surface, and multiple assays that captures the complexity of the biofilm structures. Here, Staphylococcus aureus biofilm viability, polysaccharide poly-N-acetylglucosamine, and proteins on the cell surface were analysed with agar plate- and well plate-based biofilm formation assays. Biofilms were grown on a set of nanostructured polymeric surfaces, which were thoroughly characterised for their surface chemistry and topography. Surface hydrophobicity, summit density as well as peak and valley structure were found to influence the microbial viability and exopolysaccharide abundance level in the agar plate assay. In the well plate assay, surface chemical parameters had a lesser influence on the viability, but roughness caused by valley structures increased the viability and decreased the exopolysaccharide expression. Surface proteins relating to pathogenicity were affected by the biofilm formation assay. The abundance profile of these proteins correlated clearly with several roughness parameters, especially fine structure parameters in the agar plate assay and lateral roughness in the well plate assay. These results highlight the necessity of describing the material surfaces with a versatile set of different roughness parameters to completely understand what specific features of a surface drive a certain bacterial response.

Original languageEnglish
Article number109879
JournalMaterials and Design
Volume208
DOIs
Publication statusPublished - Oct 2021
MoE publication typeA1 Journal article-refereed

Keywords

  • Biofilm formation assays
  • Physicochemical properties of surfaces
  • Roughness
  • Staphylococcus aureus
  • Surface chemistry
  • Surface proteins

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