Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings

Katriina Kurki-Helasmo; Jussi Meriluoto

doi:10.1016/S0041-0101(98)00114-7

Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings

Katriina Kurki-Helasmo
, Jussi Meriluoto^*

^*Corresponding author for this work

Laboratoryservices

Research output: Contribution to journal › Article › Scientific › peer-review

89 Citations (Scopus)

Abstract

Mustard (Sinapis alba L.) seeds were cultivated for seven days on a solid nutrient medium supplemented with 0-40μg microcystin-RR per ml. Microcystin-RR affected seedling growth (ic₅₀ 0.8μg/ml) and microcystin concentrations ≥5.0μg/ml produced malformed plants. The inhibition of protein phosphatase 1 and 2A activity correlated with the growth inhibition. The seedlings were also shown to take up ³H-dihydromicrocystin-LR derived radioactivity up to a level corresponding to ca. 80ng toxin per mg plant protein. Copyright (C) 1998 Elsevier Science Ltd.

Original language	English
Pages (from-to)	1921-1926
Number of pages	6
Journal	Toxicon
Volume	36
Issue number	12
DOIs	https://doi.org/10.1016/S0041-0101(98)00114-7
Publication status	Published - Dec 1998
MoE publication type	A1 Journal article-refereed

Funding

We thank Maj and Tor Nessling Foundation for financial support, Dr. Diana Toivola, Åbo Akademi University, for help with the toxin uptake studies and Dr. Eva-Mari Aro, Dr. Eevi Rintamäki and Dr. Juhani Lehtonen, University of Turku, for helpful discussions.

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title = "Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings",

abstract = "Mustard (Sinapis alba L.) seeds were cultivated for seven days on a solid nutrient medium supplemented with 0-40μg microcystin-RR per ml. Microcystin-RR affected seedling growth (ic50 0.8μg/ml) and microcystin concentrations ≥5.0μg/ml produced malformed plants. The inhibition of protein phosphatase 1 and 2A activity correlated with the growth inhibition. The seedlings were also shown to take up 3H-dihydromicrocystin-LR derived radioactivity up to a level corresponding to ca. 80ng toxin per mg plant protein. Copyright (C) 1998 Elsevier Science Ltd.",

author = "Katriina Kurki-Helasmo and Jussi Meriluoto",

note = "Funding Information: We thank Maj and Tor Nessling Foundation for financial support, Dr. Diana Toivola, {\AA}bo Akademi University, for help with the toxin uptake studies and Dr. Eva-Mari Aro, Dr. Eevi Rintam{\"a}ki and Dr. Juhani Lehtonen, University of Turku, for helpful discussions. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1998",

month = dec,

doi = "10.1016/S0041-0101(98)00114-7",

language = "English",

volume = "36",

pages = "1921--1926",

journal = "Toxicon",

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TY - JOUR

T1 - Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings

AU - Kurki-Helasmo, Katriina

AU - Meriluoto, Jussi

N1 - Funding Information: We thank Maj and Tor Nessling Foundation for financial support, Dr. Diana Toivola, Åbo Akademi University, for help with the toxin uptake studies and Dr. Eva-Mari Aro, Dr. Eevi Rintamäki and Dr. Juhani Lehtonen, University of Turku, for helpful discussions. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1998/12

Y1 - 1998/12

N2 - Mustard (Sinapis alba L.) seeds were cultivated for seven days on a solid nutrient medium supplemented with 0-40μg microcystin-RR per ml. Microcystin-RR affected seedling growth (ic50 0.8μg/ml) and microcystin concentrations ≥5.0μg/ml produced malformed plants. The inhibition of protein phosphatase 1 and 2A activity correlated with the growth inhibition. The seedlings were also shown to take up 3H-dihydromicrocystin-LR derived radioactivity up to a level corresponding to ca. 80ng toxin per mg plant protein. Copyright (C) 1998 Elsevier Science Ltd.

AB - Mustard (Sinapis alba L.) seeds were cultivated for seven days on a solid nutrient medium supplemented with 0-40μg microcystin-RR per ml. Microcystin-RR affected seedling growth (ic50 0.8μg/ml) and microcystin concentrations ≥5.0μg/ml produced malformed plants. The inhibition of protein phosphatase 1 and 2A activity correlated with the growth inhibition. The seedlings were also shown to take up 3H-dihydromicrocystin-LR derived radioactivity up to a level corresponding to ca. 80ng toxin per mg plant protein. Copyright (C) 1998 Elsevier Science Ltd.

UR - https://www.scopus.com/pages/publications/0032400792

U2 - 10.1016/S0041-0101(98)00114-7

DO - 10.1016/S0041-0101(98)00114-7

M3 - Article

C2 - 9839676

AN - SCOPUS:0032400792

SN - 0041-0101

VL - 36

SP - 1921

EP - 1926

JO - Toxicon

JF - Toxicon

IS - 12

ER -

Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings

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