Mesoporous silica nanoparticle-based substrates for cell directed delivery of Notch signalling modulators to control myoblast differentiation

Dominique Böcking, Oliver Wiltschka, Jenni Niinimäki, Hussein Shokry, Rolf Brenner, Mika Lindén, Cecilia Sahlgren

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)

Abstract

Biochemical cues are critical to control stem cell function and can be utilized to develop smart biomaterials for stem cell engineering. The challenge is to deliver these cues in a restricted manner with spatial and temporal control. Here we have developed bilayer films of mesoporous silica nanoparticles for delayed cellular delivery of Notch modulators to promote muscle stem cell differentiation. We demonstrate that drug-loaded particles are internalized from the particle-covered surface, which allows for direct delivery of the drug into the cell and a delayed and confined drug release. Substrates of particles loaded with gamma-secretase-inhibitors, which block the Notch signalling pathway, promoted efficient differentiation of myoblasts. The particle substrates were fully biocompatible and did not interfere with the inherent differentiation process. We further demonstrate that impregnating commercially available, biocompatible polymer scaffolds with MSNs allows for a free standing substrate for cell directed drug delivery.
Original languageUndefined/Unknown
Pages (from-to)1490–1498
Number of pages9
JournalNanoscale
Volume6
Issue number3
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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