Mass spectrometric detection of nodularin and desmethylnodularin in mussels and flounders

Krister Karlsson, Vesa Sipiä, Harri Kankaanpää, Jussi Meriluoto*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

56 Citations (Scopus)

Abstract

Samples of mussels and flounders from the Baltic Sea were analysed for nodularin content on two different LC-MS instruments (triple quadrupole and ion trap). The triple quadrupole instrument was well suited for the quantitative analysis. The limit of detection in the selected ion recording mode was 5 pg and in the multiple reactant monitoring mode 500 pg on column for extracts of Nodularia spumigena. The fragmentation patterns of nodularin-R and desmethylnodularin-R were recorded and shown to be similar to those of microcystins. LC-MS proved to be an excellent tool for the analyses of cyanobacterial hepatotoxins in complex matrices.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume784
Issue number2
DOIs
Publication statusPublished - 5 Feb 2003
MoE publication typeA1 Journal article-refereed

Funding

We wish to thank the Academy of Finland for financial support (RC for Natural Sciences and Engineering, project 47664 of J.M. and K.K., and RC for Biosciences and Environment, project 48019 of V.S.). K.K. also acknowledges the support from the National Graduate School of Informational and Structural Biology and the Magnus Ehrnrooth Foundation.

Keywords

  • Desmethylnodularin
  • Flounder
  • Mussel
  • Nodularin

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