Macrocystis pyrifera source of nutrients for the production of carotenoids by a marine yeast Rhodotorula mucilaginosa

A. Leyton, L. Flores, Päivi Mäki-Arvela, ME Lienqueo, C. Shene

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12 Citations (Scopus)

Abstract

Aims To evaluate an aqueous extract of Macrocystis pyrifera as a nutrient source for the production of carotenoids by a marine Rhodotorula mucilaginosa isolated from seaweed samples. Materials and Results The effect of different culture conditions on the concentration of biomass and total pigments was evaluated using a Box-Behnken experimental design. The seaweed extract contained 15% w w-1 of protein and 20% w w-1 of carbohydrate; the main sugar in this fraction was trehalose (78%). The culture conditions that maximize the total pigment concentration (1.84 +/- 0.03 mg l-1) were initial pH equal to 7, yeast extract as nitrogen source at a concentration of 4 g l-1, seaweed extract concentration at 25% v v-1, incubation performed at 25 degrees C and 150 rev min-1 during 6 days. Under optimal growth conditions, three carotenoids were identified among the pigments produced by R. mucilaginosa, lycopene (38.4 +/- 9.4%), beta-carotene (21.8 +/- 1.5%) and astaxanthin (1.8 +/- 0.3%). Conclusions Carotenoids of commercial interest (lycopene, beta-carotene and astaxanthin) can be produced using a marine R. mucilaginosa cultivated with an aqueous extract of M. pyrifera as nutrient source. The total pigment concentration in the culture ranged between 0.82 and 1.84 mg l-1, and was significantly affected by the concentration of the seaweed extract, and yeast extract. Significance and Impact of the Study This work demonstrates that M. pyrifera can be used as a nutrient source for the production of carotenoids by the marine yeast.
Original languageUndefined/Unknown
Pages (from-to)1069–1079
Number of pages11
JournalJournal of Applied Microbiology
Volume127
Issue number4
DOIs
Publication statusPublished - 2019
MoE publication typeA1 Journal article-refereed

Keywords

  • marine yeasts
  • seaweed

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