TY - JOUR
T1 - Layer-by-layer assembled melanin nanoparticles thin films for photodynamic activity-based disinfection by ultraviolet A irradiation
AU - Umur, Egemen
AU - Arslan, Fahriye
AU - Bakay, Emel
AU - Sirek, Busra
AU - Ayan, Bugra
AU - Baysoy, Engin
AU - Topaloğlu, Nermin
AU - Kaleli-Can, Gizem
N1 - Publisher Copyright:
© Qatar University and Springer Nature Switzerland AG 2024.
PY - 2024/12
Y1 - 2024/12
N2 - Hospitalized patients with indwelling catheters face the threat of catheter-associated infections (CAIs), which often lead to high morbidity and mortality rates. Disinfection procedures including metal-based antibacterial coatings and photosensitive nanoparticles are unsatisfactory due to concerns associated with long-term toxicity. This study investigates photodynamic capabilities of natural melanin nanoparticles (MNPs) as a layer-by-layer assembled (LBL-A) MNPs thin film to avoid biofilm formation and reveals the physical–chemical properties of LBL-A MNPs thin film using Scanning Electron Microscopy (SEM), Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy, UV–visible spectroscopy, and ability to generate reactive oxygen species (ROS). Additionally, the biocompatibility of LBL-A MNPs thin films in terms of their cytotoxic effect on fibroblast cells was examined. The highest cellular inactivation rates for E. coli bacteria with 86.7% and S. aureus with 80.5% were achieved when the developed LBL-A MNP thin films were exposed to UV-A irradiation (395–400 nm) for 60 s with a distance of 1 cm. In contrast, The LBL-A MNPs thin film protected fibroblast cells against UV-A irradiation with no significant reduction in cell viability. In this regard, the MNPs-based photodynamic method not only enables the treatment of CAIs within only 60 s of UV-A irradiation, but also eliminates the harmful effects of both UV-C and metal-based nanoparticles in living organisms.
AB - Hospitalized patients with indwelling catheters face the threat of catheter-associated infections (CAIs), which often lead to high morbidity and mortality rates. Disinfection procedures including metal-based antibacterial coatings and photosensitive nanoparticles are unsatisfactory due to concerns associated with long-term toxicity. This study investigates photodynamic capabilities of natural melanin nanoparticles (MNPs) as a layer-by-layer assembled (LBL-A) MNPs thin film to avoid biofilm formation and reveals the physical–chemical properties of LBL-A MNPs thin film using Scanning Electron Microscopy (SEM), Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy, UV–visible spectroscopy, and ability to generate reactive oxygen species (ROS). Additionally, the biocompatibility of LBL-A MNPs thin films in terms of their cytotoxic effect on fibroblast cells was examined. The highest cellular inactivation rates for E. coli bacteria with 86.7% and S. aureus with 80.5% were achieved when the developed LBL-A MNP thin films were exposed to UV-A irradiation (395–400 nm) for 60 s with a distance of 1 cm. In contrast, The LBL-A MNPs thin film protected fibroblast cells against UV-A irradiation with no significant reduction in cell viability. In this regard, the MNPs-based photodynamic method not only enables the treatment of CAIs within only 60 s of UV-A irradiation, but also eliminates the harmful effects of both UV-C and metal-based nanoparticles in living organisms.
KW - Layer-by-layer assembly
KW - Melanin nanoparticles
KW - Photodynamic disinfection
KW - UV-A irradiation
UR - http://www.scopus.com/inward/record.url?scp=85202044979&partnerID=8YFLogxK
U2 - 10.1007/s42247-024-00761-7
DO - 10.1007/s42247-024-00761-7
M3 - Article
AN - SCOPUS:85202044979
SN - 2522-5731
VL - 7
SP - 2547
EP - 2562
JO - Emergent Materials
JF - Emergent Materials
IS - 6
ER -