Projects per year
Abstract
Keratin (K) 7 is an intermediate filament protein expressed in ducts and glands of simple epithelial organs and in urothelial tissues. In the pancreas, K7 is expressed in exocrine ducts, and apico-laterally in acinar cells. Here, we report K7 expression with K8 and K18 in the endocrine islets of Langerhans in mice. K7 filament formation in islet and MIN6 β-cells is dependent on the presence and levels of K18. K18-knockout (K18‒/‒) mice have undetectable islet K7 and K8 proteins, while K7 and K18 are downregulated in K8‒/‒ islets. K7, akin to F-actin, is concentrated at the apical vertex of β-cells in wild-type mice and along the lateral membrane, in addition to forming a fine cytoplasmic network. In K8‒/‒ β-cells, apical K7 remains, but lateral keratin bundles are displaced and cytoplasmic filaments are scarce. Islet K7, rather than K8, is increased in K18 over-expressing mice and the K18-R90C mutation disrupts K7 filaments in mouse β-cells and in MIN6 cells. Notably, islet K7 filament networks significantly increase and expand in the perinuclear regions when examined in the streptozotocin diabetes model. Hence, K7 represents a significant component of the murine islet keratin network and becomes markedly upregulated during experimental diabetes.
Original language | English |
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Pages (from-to) | 1-16 |
Number of pages | 16 |
Journal | International Journal of Molecular Sciences |
Volume | 22 |
Issue number | 15 |
DOIs | |
Publication status | Published - 21 Jul 2021 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Animals
- Diabetes Mellitus, Experimental/genetics
- Gene Expression Regulation
- Insulin-Secreting Cells/metabolism
- Keratin-18/genetics
- Keratin-7/genetics
- Keratin-8/genetics
- Mice
- Mice, Knockout
- Up-Regulation
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Dive into the research topics of 'Keratin 7 Is a Constituent of the Keratin Network in Mouse Pancreatic Islets and Is Upregulated in Experimental Diabetes'. Together they form a unique fingerprint.Projects
- 2 Finished
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CellMech: Center of Excellence in Cellular Mechanostasis
Sahlgren, C. (Principal Investigator), Sistonen, L. (Principal Investigator), Eriksson, J. (Principal Investigator), Toivola, D. (Principal Investigator), Meinander, A. (Principal Investigator), Cheng, F. (Principal Investigator) & Jacquemet, G. (Principal Investigator)
01/03/19 → 29/02/24
Project: Foundation
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Keratin stress proteins as novel beta-cell health protectors and insulin regulators
Toivola, D. (Principal Investigator)
01/01/18 → 31/12/20
Project: Foundation