TY - JOUR
T1 - Ion release, hydroxyapatite conversion, and cytotoxicity of boron-containing bioactive glass scaffolds
AU - Balasubramanian, Preethi
AU - Grünewald, Alina
AU - Detsch, Rainer
AU - Hupa, Leena
AU - Jokic, Bojan
AU - Tallia, Francesca
AU - Solanki, Anu K.
AU - Jones, Julian R.
AU - Boccaccini, Aldo R.
N1 - ook
PY - 2016
Y1 - 2016
N2 - We report the development and characterization of boron-releasing highly porous three-dimensional bioactive glass (BG) scaffolds fabricated by the foam replica technique. Three types of bioactive glasses with (wt%) 0.2%, 12.5%, 25% B2O3, and related varying SiO2 contents (wt%): 50%, 37.5%, and 25%, were investigated. The well-known 13-93 (silicate) and 13-93B3 (borate) (in wt% – 56.6% B2O3, 5.5% Na2O, 11.1% K2O, 4.6% MgO, 18.5% CaO, 3.7% P2O5) BGs were used as controls to study the influence of the presence of boron on the mechanical properties, surface reactivity, and cytotoxicity of scaffolds. Surface morphology and surface properties of the BG scaffolds were measured. X-ray diffraction (XRD) analyses showed that the scaffolds of all five compositions were amorphous. The scaffolds with 12.5 wt% B2O3 exhibited satisfactory compressive strength in the range of 1–2 MPa. A dissolution study in cell culture medium was carried out, and ion release profiles, and apatite formation of the scaffolds were assessed. The cytotoxicity of the scaffolds was evaluated using a stromal cell line (ST2). Cells were found to attach and spread well on the scaffolds' surfaces. We conclude that borosilicate scaffolds containing 12.5 wt% B2O3 provide the best combination of properties, including relatively high mechanical strength, apatite formation, and cytocompatibility, and thus, they are promising candidates for bone tissue engineering.
AB - We report the development and characterization of boron-releasing highly porous three-dimensional bioactive glass (BG) scaffolds fabricated by the foam replica technique. Three types of bioactive glasses with (wt%) 0.2%, 12.5%, 25% B2O3, and related varying SiO2 contents (wt%): 50%, 37.5%, and 25%, were investigated. The well-known 13-93 (silicate) and 13-93B3 (borate) (in wt% – 56.6% B2O3, 5.5% Na2O, 11.1% K2O, 4.6% MgO, 18.5% CaO, 3.7% P2O5) BGs were used as controls to study the influence of the presence of boron on the mechanical properties, surface reactivity, and cytotoxicity of scaffolds. Surface morphology and surface properties of the BG scaffolds were measured. X-ray diffraction (XRD) analyses showed that the scaffolds of all five compositions were amorphous. The scaffolds with 12.5 wt% B2O3 exhibited satisfactory compressive strength in the range of 1–2 MPa. A dissolution study in cell culture medium was carried out, and ion release profiles, and apatite formation of the scaffolds were assessed. The cytotoxicity of the scaffolds was evaluated using a stromal cell line (ST2). Cells were found to attach and spread well on the scaffolds' surfaces. We conclude that borosilicate scaffolds containing 12.5 wt% B2O3 provide the best combination of properties, including relatively high mechanical strength, apatite formation, and cytocompatibility, and thus, they are promising candidates for bone tissue engineering.
U2 - 10.1111/ijag.12206
DO - 10.1111/ijag.12206
M3 - Artikel
SN - 2041-1286
VL - 7
SP - 206
EP - 215
JO - International Journal of Applied Glass Science
JF - International Journal of Applied Glass Science
IS - 2
ER -