Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes

Chow, Weis, Kass, Holmström, John Eriksson, Orrenius

    Research output: Contribution to journalArticleScientificpeer-review

    138 Citations (Scopus)

    Abstract

    The mechanism of Fas antigen-mediated apoptosis is at present unclear. We show here that the 100,000 x g supernatant from cell lysates prepared from anti-Fas-stimulated JUR-KAT T cells, induces chromatin fragmentation in isolated nuclei with concomitant morphological changes typically seen in apoptosis. The formation of this apoptotic nuclei promoting activity (ANPA) in JURKAT T cells after Fas antigen ligation was blocked by the serine protease inhibitors, TPCK and DCI, and by the interleukin 1-beta-converting enzyme inhibitor, VAD-FMK. In addition, chromatin degradation and morphological changes mediated by the ANPA in isolated nuclei were inhibited by TPCK, but not by DCI or VAD-FMK. These results suggest that Fas-mediated apoptosis in T cells involves the activation of a cascade of proteases.
    Original languageUndefined/Unknown
    Pages (from-to)134–138
    JournalFEBS Letters
    Volume364
    Issue number2
    Publication statusPublished - 1995
    MoE publication typeA1 Journal article-refereed

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