Investigation of protein binding with all solid-state ion-selective electrodes

Alok Prabhu, Johan Bobacka, Ari Ivaska, Kalle Levon

    Research output: Contribution to journalArticleScientificpeer-review

    6 Citations (Scopus)


    A potentiometric sensor for studying charge based adsorption of proteins was created using a single-piece polyaniline-PVC ion-selective electrode (ISE). Three different ISEs, two for Na+ and one for Cl- ion determination, were studied. The Na+-ISEs consisted of a neutral calixarene-based ionophore and one with a charged carrier dinonylnapthalenesulfonic acid (DNNSA) whereas for the Cl- ISE, an anion exchanger tridodecylmethylammonium chloride (TDDMA(+)Cl(-)), was used. The Na+ ISE with DNNSA as the charged carrier was successfully able to discriminate the binding of two different proteins (bovine serum albumin and lysozyme) based on their intrinsic charge.
    Original languageUndefined/Unknown
    Pages (from-to)1887–1894
    Number of pages8
    Issue number8
    Publication statusPublished - 2013
    MoE publication typeA1 Journal article-refereed


    • Conducting polymers
    • Electrochemical biosensors
    • Ion-selective electrodes
    • Potentiometry
    • Protein adsorption

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