Inhibition of DNA binding by differential sumoylation of heat shock factors

J Anckar, V Hietakangas, Konstantin Denessiouk, DJ Thiele, Mark S. Johnson, Lea Sistonen

    Research output: Contribution to journalArticleScientificpeer-review

    80 Citations (Scopus)

    Abstract

    Covalent modification of proteins by the small ubiquitin-related modifier SUMO regulates diverse biological functions. Sumoylation usually requires a consensus tetrapeptide, through which the binding of the SUMO-conjugating enzyme Ubc9 to the target protein is directed. However, additional specificity determinants are in many cases required. To gain insights into SUMO substrate selection, we have utilized the differential sumoylation of highly similar loop structures within the DNA-binding domains of heat shock transcription factor 1 (HSF1) and HSF2. Site-specific mutagenesis in combination with molecular modeling revealed that the sumoylation specificity is determined by several amino acids near the consensus site, which are likely to present the SUMO consensus motif to Ubc9. Importantly, we also demonstrate that sumoylation of the HSF2 loop impedes HSF2 DNA-binding activity, without affecting its oligomerization. Hence, SUMO modification of the HSF2 loop contributes to HSF-specific regulation of DNA binding and broadens the concept of sumoylation in the negative regulation of gene expression.
    Original languageUndefined/Unknown
    Pages (from-to)955–964
    Number of pages10
    JournalMolecular and Cellular Biology
    Volume26
    Issue number3
    DOIs
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed

    Cite this