In Situ Coupled Electrochemical-Goniometry as a Tool to Reveal Conformational Changes of Charged Peptides

Amir Mohammad Ghafari; Sergio E. Domínguez; Ville Järvinen; Zahra Gounani; Amandine Schmit; Marika Sjöqvist; Cecilia Sahlgren; Outi M. H. Salo-Ahen; Carita Kvarnström; Luisa Torsi; Ronald Österbacka

doi:10.1002/admi.202101480

In Situ Coupled Electrochemical-Goniometry as a Tool to Reveal Conformational Changes of Charged Peptides

Amir Mohammad Ghafari
, Sergio E. Domínguez
, Ville Järvinen
, Zahra Gounani
, Amandine Schmit
, Marika Sjöqvist
, Cecilia Sahlgren
, Outi M. H. Salo-Ahen
, Carita Kvarnström
, Luisa Torsi^*
, Ronald Österbacka^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

7 Citations (Scopus)

154 Downloads (Pure)

Abstract

The opportunity to manipulate cell functions by regulating bioactive surfaces is a potentially promising approach for organic bioelectronics. Here, the tuning of the orientation of charged peptides by means of an electrical input observed via optical tensiometry is reported. A stimuli-responsive self-assembled monolayer (SAM) with specially designed charged peptides is used as a model system to switch between two separate hydrophilic states. The underwater contact angle (UCA) technique is used to measure changes in the wetting property of a dichloromethane droplet under electrical stimuli. The observed changes in the UCA of the bio-interface can be understood in terms of a change in the surface energy between the ON and OFF states. Molecular dynamics simulations in an electric field have been performed to verify the hypothesis of the orientational change of the charged peptides upon electrical stimulation. In addition, X-ray photoelectron spectroscopy (XPS) is performed to clarify the stability of the functionalized electrodes. Finally, the possibility of using such a novel switching system as a tool to characterize bioactive surfaces is discussed.

Original language	English
Article number	2101480
Journal	Advanced Materials Interfaces
Volume	9
Issue number	4
DOIs	https://doi.org/10.1002/admi.202101480
Publication status	Published - 2021
MoE publication type	A1 Journal article-refereed

Funding

The authors thank Dr. Torsten John for sharing his work on the MD force field parameters for biotinylated peptides and Dr. Kjell-Mikael Källman for valuable assistance with the electrochemical setup equipment. The authors wish to acknowledge the Finnish IT Center for Science (CSC) and the FGCI project (Finland) for computational resources. Academy of Finland projects #316881, #316882, #316883 “Spatiotemporal control of Cell Functions”, and Åbo Akademi Endowment for “Bioelectronic Activation of Cell Functions” is acknowledged for financial support. Sergio E. Domínguez acknowledges The Magnus Ehrnrooth Foundation for a postdoctoral grant 2020-2021.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1002/admi.202101480Licence: CC BY

2021 In Situ Coupled Electrochemical-Goniometry as a Tool to Reveal Conformational Changes of Charged PeptidesFinal published version, 1.32 MBLicence: CC BY

https://urn.fi/URN:NBN:fi-fe202201148629

BACE: Center of Excellence in Bioelectronic Activation of Cell Functions
Österbacka, R. (Principal Investigator), Sjöqvist, M. (Co-Principal Investigator), Sahlgren, C. (Co-Principal Investigator), Torsi, L. (Co-Principal Investigator), Lindfelt, M. (Co-Principal Investigator), Hellsten, L. (Co-Investigator), Tewari, A. (Co-Investigator), Luukkonen, A. (Co-Investigator), Martinez Klimova, E. (Co-Investigator), Schmit, A. (Co-Investigator), Eklund, A. (Co-Investigator) & Gounani, Z. (Co-Investigator)
01/03/19 → 31/12/23
Project: Foundation
SPACE: Spatiotemporal Control of Cell Functions
Österbacka, R. (Principal Investigator), Sahlgren, C. (Co-Principal Investigator), Torsi, L. (Co-Principal Investigator) & Ghafarihashjin, A. (Co-Investigator)
01/09/18 → 31/08/22
Project: Research Council of Finland/Other Research Councils

Cite this

Ghafari, A. M., Domínguez, S. E., Järvinen, V., Gounani, Z., Schmit, A., Sjöqvist, M., Sahlgren, C., Salo-Ahen, O. M. H., Kvarnström, C., Torsi, L., & Österbacka, R. (2021). In Situ Coupled Electrochemical-Goniometry as a Tool to Reveal Conformational Changes of Charged Peptides. Advanced Materials Interfaces, 9(4), Article 2101480. https://doi.org/10.1002/admi.202101480

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abstract = "The opportunity to manipulate cell functions by regulating bioactive surfaces is a potentially promising approach for organic bioelectronics. Here, the tuning of the orientation of charged peptides by means of an electrical input observed via optical tensiometry is reported. A stimuli-responsive self-assembled monolayer (SAM) with specially designed charged peptides is used as a model system to switch between two separate hydrophilic states. The underwater contact angle (UCA) technique is used to measure changes in the wetting property of a dichloromethane droplet under electrical stimuli. The observed changes in the UCA of the bio-interface can be understood in terms of a change in the surface energy between the ON and OFF states. Molecular dynamics simulations in an electric field have been performed to verify the hypothesis of the orientational change of the charged peptides upon electrical stimulation. In addition, X-ray photoelectron spectroscopy (XPS) is performed to clarify the stability of the functionalized electrodes. Finally, the possibility of using such a novel switching system as a tool to characterize bioactive surfaces is discussed.",

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AU - Gounani, Zahra

AU - Schmit, Amandine

AU - Sjöqvist, Marika

AU - Sahlgren, Cecilia

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AU - Torsi, Luisa

AU - Österbacka, Ronald

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AB - The opportunity to manipulate cell functions by regulating bioactive surfaces is a potentially promising approach for organic bioelectronics. Here, the tuning of the orientation of charged peptides by means of an electrical input observed via optical tensiometry is reported. A stimuli-responsive self-assembled monolayer (SAM) with specially designed charged peptides is used as a model system to switch between two separate hydrophilic states. The underwater contact angle (UCA) technique is used to measure changes in the wetting property of a dichloromethane droplet under electrical stimuli. The observed changes in the UCA of the bio-interface can be understood in terms of a change in the surface energy between the ON and OFF states. Molecular dynamics simulations in an electric field have been performed to verify the hypothesis of the orientational change of the charged peptides upon electrical stimulation. In addition, X-ray photoelectron spectroscopy (XPS) is performed to clarify the stability of the functionalized electrodes. Finally, the possibility of using such a novel switching system as a tool to characterize bioactive surfaces is discussed.

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In Situ Coupled Electrochemical-Goniometry as a Tool to Reveal Conformational Changes of Charged Peptides

Abstract

Funding

UN SDGs

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Projects

BACE: Center of Excellence in Bioelectronic Activation of Cell Functions

SPACE: Spatiotemporal Control of Cell Functions

Cite this