In Situ Coupled Electrochemical-Goniometry as a Tool to Reveal Conformational Changes of Charged Peptides

Amir Mohammad Ghafari, Sergio E. Domínguez, Ville Järvinen, Zahra Gounani, Amandine Schmit, Marika Sjöqvist, Cecilia Sahlgren, Outi M. H. Salo-Ahen, Carita Kvarnström, Luisa Torsi*, Ronald Österbacka*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)
40 Downloads (Pure)


The opportunity to manipulate cell functions by regulating bioactive surfaces is a potentially promising approach for organic bioelectronics. Here, the tuning of the orientation of charged peptides by means of an electrical input observed via optical tensiometry is reported. A stimuli-responsive self-assembled monolayer (SAM) with specially designed charged peptides is used as a model system to switch between two separate hydrophilic states. The underwater contact angle (UCA) technique is used to measure changes in the wetting property of a dichloromethane droplet under electrical stimuli. The observed changes in the UCA of the bio-interface can be understood in terms of a change in the surface energy between the ON and OFF states. Molecular dynamics simulations in an electric field have been performed to verify the hypothesis of the orientational change of the charged peptides upon electrical stimulation. In addition, X-ray photoelectron spectroscopy (XPS) is performed to clarify the stability of the functionalized electrodes. Finally, the possibility of using such a novel switching system as a tool to characterize bioactive surfaces is discussed.
Original languageEnglish
Article number2101480
JournalAdvanced Materials Interfaces
Issue number4
Publication statusPublished - 2021
MoE publication typeA1 Journal article-refereed


Dive into the research topics of 'In Situ Coupled Electrochemical-Goniometry as a Tool to Reveal Conformational Changes of Charged Peptides'. Together they form a unique fingerprint.

Cite this