High-performance liquid chromatographic separation of microcystins and nodularin, cyanobacterial peptide toxins, on C18 and amide C16 sorbents

Lisa Spoof; Krister Karlsson; Jussi Meriluoto

doi:10.1016/S0021-9673(00)01099-2

High-performance liquid chromatographic separation of microcystins and nodularin, cyanobacterial peptide toxins, on C₁₈ and amide C₁₆ sorbents

Lisa Spoof
, Krister Karlsson
, Jussi Meriluoto^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

59 Citations (Scopus)

Abstract

Four C₁₈ columns and a novel amide C₁₆ column were assessed in the HPLC separation of eight microcystins and nodularin-R. Gradient mobile phases of acetonitrile combined with trifluoroacetic acid, formic acid or ammonium acetate were compared. Special attention was paid to the resolution of four possible coeluting microcystin pairs. Generally speaking, the acidic mobile phases were superior to the ammonium acetate-based mobile phase in terms of resolution and selectivity. The amide C₁₆ column had the best overall performance and unique selectivity properties.

Original language	English
Pages (from-to)	225-236
Number of pages	12
Journal	Journal of Chromatography A
Volume	909
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(00)01099-2
Publication status	Published - 16 Feb 2001
MoE publication type	A1 Journal article-refereed

Funding

The authors wish to thank the Maj and Tor Nessling Foundation and the Academy of Finland for financial support. K.K. acknowledges support from the Magnus Ehrnrooth Foundation. Dr. Mikko Ora and Kristo Hakala are thanked for their help with the LC–MS experiment.

Keywords

Microcystins
Mobile phase composition
Nodularin
Stationary phases, LC
Toxins

Access to Document

10.1016/S0021-9673(00)01099-2

Cite this

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title = "High-performance liquid chromatographic separation of microcystins and nodularin, cyanobacterial peptide toxins, on C18 and amide C16 sorbents",

abstract = "Four C18 columns and a novel amide C16 column were assessed in the HPLC separation of eight microcystins and nodularin-R. Gradient mobile phases of acetonitrile combined with trifluoroacetic acid, formic acid or ammonium acetate were compared. Special attention was paid to the resolution of four possible coeluting microcystin pairs. Generally speaking, the acidic mobile phases were superior to the ammonium acetate-based mobile phase in terms of resolution and selectivity. The amide C16 column had the best overall performance and unique selectivity properties.",

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AU - Meriluoto, Jussi

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N2 - Four C18 columns and a novel amide C16 column were assessed in the HPLC separation of eight microcystins and nodularin-R. Gradient mobile phases of acetonitrile combined with trifluoroacetic acid, formic acid or ammonium acetate were compared. Special attention was paid to the resolution of four possible coeluting microcystin pairs. Generally speaking, the acidic mobile phases were superior to the ammonium acetate-based mobile phase in terms of resolution and selectivity. The amide C16 column had the best overall performance and unique selectivity properties.

AB - Four C18 columns and a novel amide C16 column were assessed in the HPLC separation of eight microcystins and nodularin-R. Gradient mobile phases of acetonitrile combined with trifluoroacetic acid, formic acid or ammonium acetate were compared. Special attention was paid to the resolution of four possible coeluting microcystin pairs. Generally speaking, the acidic mobile phases were superior to the ammonium acetate-based mobile phase in terms of resolution and selectivity. The amide C16 column had the best overall performance and unique selectivity properties.

KW - Microcystins

KW - Mobile phase composition

KW - Nodularin

KW - Stationary phases, LC

KW - Toxins

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DO - 10.1016/S0021-9673(00)01099-2

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