High-performance liquid chromatographic separation of microcystins and nodularin, cyanobacterial peptide toxins, on C18 and amide C16 sorbents

Lisa Spoof, Krister Karlsson, Jussi Meriluoto*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

57 Citations (Scopus)

Abstract

Four C18 columns and a novel amide C16 column were assessed in the HPLC separation of eight microcystins and nodularin-R. Gradient mobile phases of acetonitrile combined with trifluoroacetic acid, formic acid or ammonium acetate were compared. Special attention was paid to the resolution of four possible coeluting microcystin pairs. Generally speaking, the acidic mobile phases were superior to the ammonium acetate-based mobile phase in terms of resolution and selectivity. The amide C16 column had the best overall performance and unique selectivity properties.

Original languageEnglish
Pages (from-to)225-236
Number of pages12
JournalJournal of Chromatography A
Volume909
Issue number2
DOIs
Publication statusPublished - 16 Feb 2001
MoE publication typeA1 Journal article-refereed

Keywords

  • Microcystins
  • Mobile phase composition
  • Nodularin
  • Stationary phases, LC
  • Toxins

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