GFAP isoforms control intermediate filament network dynamics, cell morphology, and focal adhesions.

M Moeton, Oscar Stassen, JA Sluijs, van der Meer VWN, LJ Kluivers, van Hoorn H, T Schmidt, Reits EAJ, van Strien ME, EM Hol

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

Glial fibrillary acidic protein (GFAP) is the characteristic intermediate filament (IF) protein in astrocytes. Expression of its main isoforms, GFAPα and GFAPδ, varies in astrocytes and astrocytoma implying a potential regulatory role in astrocyte physiology and pathology. An IF-network is a dynamic structure and has been functionally linked to cell motility, proliferation, and morphology. There is a constant exchange of IF-proteins with the network. To study differences in the dynamic properties of GFAPα and GFAPδ, we performed fluorescence recovery after photobleaching experiments on astrocytoma cells with fluorescently tagged GFAPs. Here, we show for the first time that the exchange of GFP-GFAPδ was significantly slower than the exchange of GFP-GFAPα with the IF-network. Furthermore, a collapsed IF-network, induced by GFAPδ expression, led to a further decrease in fluorescence recovery of both GFP-GFAPα and GFP-GFAPδ. This altered IF-network also changed cell morphology and the focal adhesion size, but did not alter cell migration or proliferation. Our study provides further insight into the modulation of the dynamic properties and functional consequences of the IF-network composition.
Original languageUndefined/Unknown
Pages (from-to)4101–4120
JournalCellular and Molecular Life Sciences
Volume73
Issue number21
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Keywords

  • intermediate filaments
  • FRAP
  • GFAP
  • astrocytoma

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