Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering

O Blazevits, YG Mideksa, M Solman, A Ligabue, N Ariotti, H Nakhaeizadeh, EK Fansa, AC Papageorgiou, A Wittinghofer, MR Ahmadian, Daniel Abankwa

Research output: Contribution to journalArticleScientificpeer-review

36 Citations (Scopus)

Abstract

Galectin-1 (Gal-1) dimers crosslink carbohydrates on cell surface receptors. Carbohydrate-derived inhibitors have been developed for cancer treatment. Intracellularly, Gal-1 was suggested to interact with the farnesylated C-terminus of Ras thus specifically stabilizing GTP-H-ras nanoscale signalling hubs in the membrane, termed nanoclusters. The latter activity may present an alternative mechanism for how overexpressed Gal-1 stimulates tumourigenesis. Here we revise the current model for the interaction of Gal-1 with H-ras. We show that it indirectly forms a complex with GTP-H-ras via a high-affinity interaction with the Ras binding domain (RBD) of Ras effectors. A computationally generated model of the Gal-1/C-Raf-RBD complex is validated by mutational analysis. Both cellular FRET as well as proximity ligation assay experiments confirm interaction of Gal-1 with Raf proteins in mammalian cells. Consistently, interference with H-rasG12V-effector interactions basically abolishes H-ras nanoclustering. In addition, an intact dimer interface of Gal-1 is required for it to positively regulate H-rasG12V nanoclustering, but negatively K-rasG12V nanoclustering. Our findings suggest stacked dimers of H-ras, Raf and Gal-1 as building blocks of GTP-H-ras-nanocluster at high Gal-1 levels. Based on our results the Gal-1/effector interface represents a potential drug target site in diseases with aberrant Ras signalling.
Original languageUndefined/Unknown
Pages (from-to)1–16
Number of pages16
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

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