Functional consequences of knocking down porcine prostaglandin synthases in SK-6 swine kidney cell line

A Waclawik, Adolfo Rivero Müller, NA Rahman, AJ Ziecik

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

We hypothesized that in vitro knock-down of the previously cloned genes of prostaglandin synthases will result in a reduction of synthesis, and thus secretion of their respective products, i.e., prostaglandin (PG) E-2 or PGF(2 alpha). For this purpose, we designed short hairpin RNA (shRNA)-encoding constructs to knock down porcine mPGES-1 and PGFS (also named as AKR1CL1 in GenBank) and used them to transfect swine kidney SK-6 cells. Knocking down PGFS or mPGES-1 transcripts resulted in at least 50% inhibition of protein expression of each respective enzyme, as well as a reduction in the production of their respective prostaglandin, PGF(2 alpha), or PGE(2). These results confirmed the identities of PGFS and mPGES-1. Moreover, they illustrate a unique opportunity to use the gene knock-down constructs in primary endometrial cells in order to study their biological roles in the porcine endometrium, particularly during the establishment of pregnancy.
Original languageUndefined/Unknown
Pages (from-to)42–47
Number of pages6
JournalReproductive Biology -Olsztyn-
Volume15
Issue number1
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • Gene knock-down
  • Pig
  • Prostaglandins
  • ShRNA
  • SK-6 cells

Cite this