TY - JOUR
T1 - Fluid dynamics modeling for synchronizing surface plasmon resonance and quartz crystal microbalance as tools for biomolecular and targeted drug delivery studies
AU - Viitala, Tapani
AU - Liang, Huamin
AU - Gupta, Mayur
AU - Zwinger, Thomas
AU - Yliperttula, Marjo
AU - Bunker, Alex
N1 - Funding Information:
We thank Jussi-Pekka Tuppurainen from Bionavis Ltd., and Niko Granqvist for valuable discussions during this study. Dr. Lasse Murtomäki, Teemu Laurila, and Terhi Laurila are acknowledged for their help with Comsol modeling. TV and HL acknowledge Academy of Finland for financial support (Grant Nos. 137053 and 140980). AB acknowledges support from CIMO.
PY - 2012/7/15
Y1 - 2012/7/15
N2 - We have used computational fluid dynamics modeling (CFD) to synchronize the flow conditions in the flow channels of two complementary surface-sensitive characterization techniques: surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). Since the footprint of the flow channels of the two devices is specified by their function, the flow behavior can only be varied either by altering the height of the flow channel, or altering the volumetric rate of flow (flow rate) through the channel. The relevant quantity that must be calibrated is the shear strain on the measurement surface (center and bottom) of the flow channel. Our CFD modeling shows that the flow behavior is in the Stokes flow regime. We were thus able to generate a scaling expression with parameters for flow rate and flow channel height for each of the two devices: f QCM=2.64f SPR(h QCM/h SPR) 2, where f QCM and f SPR are the flow rates in the SPR and QCM flow channels, respectively, and h QCM/h SPR is the ratio of the heights of the two channels. We demonstrate the success of our calibration procedure through the combined use of commercially available SPR and QCM flow channel devices on both a biomolecular interaction system of surface immobilized biotin and streptavidin and a targeted drug delivery model system of biotinylated liposomes interacting with a streptavidin functionalized surface.
AB - We have used computational fluid dynamics modeling (CFD) to synchronize the flow conditions in the flow channels of two complementary surface-sensitive characterization techniques: surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). Since the footprint of the flow channels of the two devices is specified by their function, the flow behavior can only be varied either by altering the height of the flow channel, or altering the volumetric rate of flow (flow rate) through the channel. The relevant quantity that must be calibrated is the shear strain on the measurement surface (center and bottom) of the flow channel. Our CFD modeling shows that the flow behavior is in the Stokes flow regime. We were thus able to generate a scaling expression with parameters for flow rate and flow channel height for each of the two devices: f QCM=2.64f SPR(h QCM/h SPR) 2, where f QCM and f SPR are the flow rates in the SPR and QCM flow channels, respectively, and h QCM/h SPR is the ratio of the heights of the two channels. We demonstrate the success of our calibration procedure through the combined use of commercially available SPR and QCM flow channel devices on both a biomolecular interaction system of surface immobilized biotin and streptavidin and a targeted drug delivery model system of biotinylated liposomes interacting with a streptavidin functionalized surface.
KW - Biomolecular interaction
KW - Biotin
KW - Drug delivery
KW - Finite Element method
KW - Hydrodynamic modeling
KW - Liposome
KW - Quartz crystal microbalance
KW - Shear stress
KW - Streptavidin
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=84861580133&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2012.04.012
DO - 10.1016/j.jcis.2012.04.012
M3 - Article
C2 - 22579516
AN - SCOPUS:84861580133
SN - 0021-9797
VL - 378
SP - 251
EP - 259
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
IS - 1
ER -