Fluid dynamics modeling for synchronizing surface plasmon resonance and quartz crystal microbalance as tools for biomolecular and targeted drug delivery studies

Tapani Viitala, Huamin Liang, Mayur Gupta, Thomas Zwinger, Marjo Yliperttula, Alex Bunker*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We have used computational fluid dynamics modeling (CFD) to synchronize the flow conditions in the flow channels of two complementary surface-sensitive characterization techniques: surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). Since the footprint of the flow channels of the two devices is specified by their function, the flow behavior can only be varied either by altering the height of the flow channel, or altering the volumetric rate of flow (flow rate) through the channel. The relevant quantity that must be calibrated is the shear strain on the measurement surface (center and bottom) of the flow channel. Our CFD modeling shows that the flow behavior is in the Stokes flow regime. We were thus able to generate a scaling expression with parameters for flow rate and flow channel height for each of the two devices: f QCM=2.64f SPR(h QCM/h SPR) 2, where f QCM and f SPR are the flow rates in the SPR and QCM flow channels, respectively, and h QCM/h SPR is the ratio of the heights of the two channels. We demonstrate the success of our calibration procedure through the combined use of commercially available SPR and QCM flow channel devices on both a biomolecular interaction system of surface immobilized biotin and streptavidin and a targeted drug delivery model system of biotinylated liposomes interacting with a streptavidin functionalized surface.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalJournal of Colloid and Interface Science
Volume378
Issue number1
DOIs
Publication statusPublished - 15 Jul 2012
Externally publishedYes
MoE publication typeA1 Journal article-refereed

Keywords

  • Biomolecular interaction
  • Biotin
  • Drug delivery
  • Finite Element method
  • Hydrodynamic modeling
  • Liposome
  • Quartz crystal microbalance
  • Shear stress
  • Streptavidin
  • Surface plasmon resonance

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