Abstract
Six modern narrow-bore (50 mm length × 2-2.1 mm internal diameter) reversed-phase HPLC columns were tested in the rapid separation of ten microcystins and three nodularins, cyanobacterial peptide hepatotoxins. The columns had either a sub-3 μm particle size or were of monolithic silica technology for high efficiency and rapid run times. A standard low-pressure gradient HPLC equipment with diode-array UV detector was used for the chromatography. The gradient mobile phase consisted of water and acetonitrile, both acidified with trifluoroacetic acid, and the gradient rise times were 1-4 min. Chromatographic performance was assessed by visual judgement and by calculating parameters such as capacity factors, resolution, peak width, selectivity and peak form. Several chromatographic conditions produced excellent results. We recommend sub-3 μm particle-based or monolithic reversed-phase columns for routine use in microcystin and nodularin HPLC analyses.
| Original language | English |
|---|---|
| Pages (from-to) | 954-964 |
| Number of pages | 11 |
| Journal | Toxicon |
| Volume | 55 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2010 |
| MoE publication type | A1 Journal article-refereed |
Funding
The authors wish to thank Tekes (decision number 40366/06) and the Academy of Finland (decision number 108947) for financial support. M.-R.N. thanks the National Graduate School in Informational and Structural Biology for support. Dr. Tomasz Jurczak, University of Lodz, is thanked for the cyanobacterial bloom material collected from Sulejow Reservoir.
Keywords
- Fast high-performance liquid chromatography
- Microcystins
- Nodularins