TY - JOUR
T1 - Fast separation of microcystins and nodularins on narrow-bore reversed-phase columns coupled to a conventional HPLC system
AU - Spoof, Lisa
AU - Neffling, Milla Riina
AU - Meriluoto, Jussi
N1 - Funding Information:
The authors wish to thank Tekes (decision number 40366/06) and the Academy of Finland (decision number 108947) for financial support. M.-R.N. thanks the National Graduate School in Informational and Structural Biology for support. Dr. Tomasz Jurczak, University of Lodz, is thanked for the cyanobacterial bloom material collected from Sulejow Reservoir.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/5
Y1 - 2010/5
N2 - Six modern narrow-bore (50 mm length × 2-2.1 mm internal diameter) reversed-phase HPLC columns were tested in the rapid separation of ten microcystins and three nodularins, cyanobacterial peptide hepatotoxins. The columns had either a sub-3 μm particle size or were of monolithic silica technology for high efficiency and rapid run times. A standard low-pressure gradient HPLC equipment with diode-array UV detector was used for the chromatography. The gradient mobile phase consisted of water and acetonitrile, both acidified with trifluoroacetic acid, and the gradient rise times were 1-4 min. Chromatographic performance was assessed by visual judgement and by calculating parameters such as capacity factors, resolution, peak width, selectivity and peak form. Several chromatographic conditions produced excellent results. We recommend sub-3 μm particle-based or monolithic reversed-phase columns for routine use in microcystin and nodularin HPLC analyses.
AB - Six modern narrow-bore (50 mm length × 2-2.1 mm internal diameter) reversed-phase HPLC columns were tested in the rapid separation of ten microcystins and three nodularins, cyanobacterial peptide hepatotoxins. The columns had either a sub-3 μm particle size or were of monolithic silica technology for high efficiency and rapid run times. A standard low-pressure gradient HPLC equipment with diode-array UV detector was used for the chromatography. The gradient mobile phase consisted of water and acetonitrile, both acidified with trifluoroacetic acid, and the gradient rise times were 1-4 min. Chromatographic performance was assessed by visual judgement and by calculating parameters such as capacity factors, resolution, peak width, selectivity and peak form. Several chromatographic conditions produced excellent results. We recommend sub-3 μm particle-based or monolithic reversed-phase columns for routine use in microcystin and nodularin HPLC analyses.
KW - Fast high-performance liquid chromatography
KW - Microcystins
KW - Nodularins
UR - http://www.scopus.com/inward/record.url?scp=77649232476&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2009.06.015
DO - 10.1016/j.toxicon.2009.06.015
M3 - Article
C2 - 19540867
AN - SCOPUS:77649232476
SN - 0041-0101
VL - 55
SP - 954
EP - 964
JO - Toxicon
JF - Toxicon
IS - 5
ER -