TY - JOUR
T1 - Fast and Reliable Electronic Assay of a Xylella fastidiosa Single Bacterium in Infected Plants Sap
AU - Sarcina, Lucia
AU - Macchia, Eleonora
AU - Loconsole, Giuliana
AU - D'Attoma, Giusy
AU - Bollella, Paolo
AU - Catacchio, Michele
AU - Leonetti, Francesco
AU - Di Franco, Cinzia
AU - Elicio, Vito
AU - Scamarcio, Gaetano
AU - Palazzo, Gerardo
AU - Boscia, Donato
AU - Saldarelli, Pasquale
AU - Torsi, Luisa
N1 - Publisher Copyright:
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
PY - 2022/10/25
Y1 - 2022/10/25
N2 - Pathogens ultra-sensitive detection is vital for early diagnosis and provision of restraining actions and/or treatments. Among plant pathogens, Xylella fastidiosa is among the most threatening as it can infect hundreds of plant species worldwide with consequences on agriculture and the environment. An electrolyte-gated transistor is here demonstrated to detect X. fastidiosa at a limit-of-quantification (LOQ) of 2 ± 1 bacteria in 0.1 mL (20 colony-forming-unit per mL). The assay is carried out with a millimeter-wide gate functionalized with Xylella-capturing antibodies directly in saps recovered from naturally infected plants. The proposed platform is benchmarked against the quantitave polymerase chain reaction (qPCR) gold standard, whose LOQ turns out to be at least one order of magnitude higher. Furthermore, the assay selectivity is proven against the Paraburkholderia phytofirmans bacterium (negative-control experiment). The proposed label-free, fast (30 min), and precise (false-negatives, false-positives below 1%) electronic assay, lays the ground for an ultra-high performing immunometric point-of-care platform potentially enabling large-scale screening of asymptomatic plants.
AB - Pathogens ultra-sensitive detection is vital for early diagnosis and provision of restraining actions and/or treatments. Among plant pathogens, Xylella fastidiosa is among the most threatening as it can infect hundreds of plant species worldwide with consequences on agriculture and the environment. An electrolyte-gated transistor is here demonstrated to detect X. fastidiosa at a limit-of-quantification (LOQ) of 2 ± 1 bacteria in 0.1 mL (20 colony-forming-unit per mL). The assay is carried out with a millimeter-wide gate functionalized with Xylella-capturing antibodies directly in saps recovered from naturally infected plants. The proposed platform is benchmarked against the quantitave polymerase chain reaction (qPCR) gold standard, whose LOQ turns out to be at least one order of magnitude higher. Furthermore, the assay selectivity is proven against the Paraburkholderia phytofirmans bacterium (negative-control experiment). The proposed label-free, fast (30 min), and precise (false-negatives, false-positives below 1%) electronic assay, lays the ground for an ultra-high performing immunometric point-of-care platform potentially enabling large-scale screening of asymptomatic plants.
KW - Electrolyte gate organic field-effect transistor
KW - Single bacterium sensing
KW - Single-molecule assay with a large transistor
UR - http://www.scopus.com/inward/record.url?scp=85137002397&partnerID=8YFLogxK
U2 - 10.1002/advs.202203900
DO - 10.1002/advs.202203900
M3 - Article
C2 - 36031404
AN - SCOPUS:85137002397
SN - 2198-3844
VL - 9
JO - Advanced Science
JF - Advanced Science
IS - 30
M1 - 2203900
ER -