Facile fractionation of bamboo hydrolysate and characterization of isolated lignin and lignin-carbohydrate complexes

Xiaodi Wang, Yongchao Zhang, Luyao Wang, Xiaoju Wang, Qingxi Hou*, Stefan Willför, Yingjuan Fu, Menghua Qin, Chunlin Xu

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

An efficient separation technology for hydrolysates towards a full valorization of bamboo is still a tough challenge, especially regarding the lignin and lignin-carbohydrate complexes (LCCs). The present study aimed to develop a facile approach using organic solvent extraction for efficiently fractionating the main components of bamboo hydrolysates. The high-purity lignin with only a trace of carbohydrates was first obtained by precipitation of the bamboo hydrolysate. The water-soluble lignin (WSL) fraction was extracted in organic solvent through a three-stage organic solvent extraction process, and the hemicellulosic sugars with increased purity were also collected. Furthermore, a thorough characterization including various NMR techniques (31P, 13C, and 2D-HSQC), GPC, and GC-MS was conducted to the obtained lignin-rich-fractions. It was found that the WSL fraction contained abundant functional groups and tremendous amount of LCC structures. As compared to native LCC of bamboo, the WSL fraction exhibited more typical LCC linkages, i.e. phenyl glycoside linkage, which is the main type of chemical linkage between lignin and carbohydrate in both LCC samples. The results demonstrate that organic phase extraction is a highly efficient protocol for the fractionation of hydrolysate and the isolation of LCC-rich streams possessing great potential applications.
Original languageEnglish
JournalHolzforschung
DOIs
Publication statusE-pub ahead of print - 14 Aug 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • hydrolysate
  • lignin
  • lignin-carbohydrate complexes (LCCs)
  • organic solvent fractionation

Fingerprint Dive into the research topics of 'Facile fractionation of bamboo hydrolysate and characterization of isolated lignin and lignin-carbohydrate complexes'. Together they form a unique fingerprint.

Cite this