Fabrication of 3D dual-layered nanofibrous graft loaded with layered double hydroxides and their effects in osteoblastic behavior for bone tissue engineering

Nanofibers coated onto the bone implant as a dual-layered material has supported the regeneration of bone cells suitable for bone tissue engineering. Herein, we report the electrospinning of Poly(3-Hydroxybutyric acid)-Poly(N-vinylpyrrolidone) NanoFibrous Matrix (NFM) loaded with Layered Double Hydroxides (LDH) was coated over the Hydroxyapatite (HAP) pellet as a dual-layered nanofibrous bone graft for bone tissue engineering. The morphology of the fabricated dual-layered nanofibrous bone graft was characterized using scanning electron microscopy. Further, the existence of the LDH and HAP over the dual layer were analyzed with EDX spectrum. The in vitro biocompatibility and fluorescence activity of the dual −layered nanofibrous bone graft (NFM-LDH: HAP) against MG63 cell lines was studied over 1, 3, 7 and 14 days. The osteoblast like MG63 cells assisted excellent cell adhesion and proliferation over both top and bottom layer of the dual-layered nanofibrous bone graft. In conclusion, the fabricated bone graft proved to be promising bone implant in tissue engineering application.