Exploring Alternative Radiolabeling Strategies for Sialic Acid-Binding Immunoglobulin-Like Lectin 9 Peptide: [68Ga]Ga- and [18F]AlF-NOTA-Siglec-9

Xiang-Guo Li, Riikka Siitonen, Heidi Liljenbäck, Elli Suomela, Sirpa Jalkanen, Li Xiang-Guo, Anne Roivainen

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Amino acid residues 283–297 from sialic acid-binding immunoglobulin-like lectin 9 (Siglec-9) form a cyclic peptide ligand targeting vascular adhesion protein-1 (VAP-1). VAP-1 is associated with the transfer of leukocytes from blood to tissues upon inflammation. Therefore, analogs of Siglec-9 peptide are good candidates for visualizing inflammation non-invasively using positron emission tomography (PET). Gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N,N′,N″,N‴-tetraacetic acid (DOTA)-conjugated Siglec-9 has been evaluated extensively for this purpose. Here, we explored two alternative strategies for radiolabeling Siglec-9 peptide using a 1,4,7-triazacyclononane-triacetic acid (NOTA)-chelator to bind [68Ga]Ga or [18F]AlF. The radioligands were evaluated by in vivo PET imaging and ex vivo γ-counting of turpentine-induced sterile skin/muscle inflammation in Sprague-Dawley rats. Both tracers showed clear accumulation in the inflamed tissues. The whole-body biodistribution patterns of the tracers were similar.

Keywords: aluminum fluoride, fluorine-18, gallium-68, inflammation, positron emission tomography, Siglec-9, VAP-1
Original languageUndefined/Unknown
Pages (from-to)
JournalMolecules
Volume23
Issue number305
DOIs
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed

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