Aqueous size-exclusion chromatography (SEC) equipped with refractive index (RI) and multiangle laser light scattering (MALLS) detectors were applied to evaluation of charged molecules content in a series of polysaccharide derivatives with different average molar mass (MM) values. It was shown that a minor amount of residual charged groups in the polysaccharide chains caused a polyelectrolyte expansion effect and neutral fractions of birch xylans, larch arabinogalactans (AG) and galactoglucomannans (GGM) were separated from neutral molecules in various concentrations of the injected samples.
|Journal||Sorbcionnye i hromatograficheskie processy|
|Publication status||Published - 2017|
|MoE publication type||A1 Journal article-refereed|
- size exclusion chromatography
- molar mass
- polyelectrolyte expansion
- concentration effects