Evaluation of acid methanolysis for analysis of wood hemicelluloses and pectins

F Bertaud, Anna Sundberg, Bjarne Holmbom

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    76 Citations (Scopus)

    Abstract

    The efficiency of acid methanolysis toward cleavage of glucuronosyl linkages in hemicelluloses and pectins was studied. Two aldo-uronic acids (a dimer and a tetramer) were submitted to methanolysis with different acidic strength and methanolysis time. The aldo-uronic acid tetramer was only affected by the acidity of the medium, whereas the dimer was sensitive towards both reaction time and acidity. It was concluded that methanolysis of 0.5-1 mg of model compounds with 2 ml of 2 M HCl/MeOH at 100degreesC for 3-5 h was the best compromise between good cleavage of the glucuronosyl bonds ( approximate to 70%, mol/mol) and little degradation of the uronic acid released (below 20%, mol/ mol). The acid methanolysis of reference oligo- and polysaccharides verified the good accuracy of the method. The application of acid methanolysis on mechanical pulp fibres was tested on a thermomechanical pulp. (C) 2002 Elsevier Science Ltd. All rights reserved.
    Original languageUndefined/Unknown
    Pages (from-to)319–324
    Number of pages6
    JournalCarbohydrate Polymers
    Volume48
    Issue number3
    Publication statusPublished - 2002
    MoE publication typeA1 Journal article-refereed

    Keywords

    • fibres
    • glucuronosyl linkage
    • hemicelluloses
    • methanolysis
    • pectins
    • polysaccharide
    • Pulp
    • uronic acid
    • wood

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