Enhanced antibody responses in CD19-Cre mice

Diogo M. Cunha; Sara Hernández-Pérez; Pieta K. Mattila

doi:10.1038/s41598-024-83954-8

Enhanced antibody responses in CD19-Cre mice

Diogo M. Cunha
, Sara Hernández-Pérez
, Pieta K. Mattila^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

2 Citations (Scopus)

30 Downloads (Pure)

Abstract

CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.

Original language	English
Article number	1348
Journal	Scientific Reports
Volume	15
Issue number	1
DOIs	https://doi.org/10.1038/s41598-024-83954-8
Publication status	Published - 8 Jan 2025
MoE publication type	A1 Journal article-refereed

Funding

We are thankful to Laura Grönfors, M. Özge Balci and Chiara Urban for technical assistance. Turku Bioscience Cell Imaging and Cytometry Core and Biocenter Finland are acknowledged for providing research infrastructures. This work was supported by the Research Council of Finland’s Flagship InFLAMES (decision numbers: 337530 and 357910) and project funding (decision numbers: 296684, 327378, and 339810 to P.K.M.), and the Sigrid Jusélius (to P.K.M.) and Finnish Cultural Foundations (D.M.C. and S.H-P).

Keywords

Antibody response
B cells
BCR
CD19
Class-switch recombination

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10.1038/s41598-024-83954-8

s41598-024-83954-8Final published version, 3.2 MBLicence: CC BY-NC-ND

https://urn.fi/URN:NBN:fi-fe20251128112490

Cite this

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title = "Enhanced antibody responses in CD19-Cre mice",

abstract = "CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.",

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AU - Cunha, Diogo M.

AU - Hernández-Pérez, Sara

AU - Mattila, Pieta K.

N1 - Publisher Copyright: © The Author(s) 2025.

PY - 2025/1/8

Y1 - 2025/1/8

N2 - CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.

AB - CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.

KW - Antibody response

KW - B cells

KW - BCR

KW - CD19

KW - Class-switch recombination

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Enhanced antibody responses in CD19-Cre mice

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