Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

Jussi Meriluoto; Brendan Kincaid; Malcolm R Smyth; Mikael Wasberg

doi:10.1016/S0021-9673(98)00203-9

Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

Jussi Meriluoto^*, Brendan Kincaid, Malcolm R Smyth, Mikael Wasberg

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

39 Citations (Scopus)

Abstract

A novel amperometric HPLC detection method for the cyanobacterial (blue- green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins.

Original language	English
Pages (from-to)	226-230
Number of pages	5
Journal	Journal of Chromatography A
Volume	810
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(98)00203-9
Publication status	Published - 12 Jun 1998
MoE publication type	A1 Journal article-refereed

Funding

We thank Dr. Tore Lindholm for the field sample, Dr. Kaarina Sivonen for the original Anabaena material, Mrs. Katriina Kurki-Helasmo for growing the cyanobacteria, Mr. Markku Reunanen for the electrospray MS in the characterization of the purified toxins and Mr. Allan Nurmi for technical assistance. B.K. thanks the Erasmus scheme for a travel grant.

Keywords

Cyanobacteria
Detection, LC
Hepatotoxins
Microcystins
Toxins

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10.1016/S0021-9673(98)00203-9

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title = "Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography",

abstract = "A novel amperometric HPLC detection method for the cyanobacterial (blue- green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins.",

keywords = "Cyanobacteria, Detection, LC, Hepatotoxins, Microcystins, Toxins",

author = "Jussi Meriluoto and Brendan Kincaid and Smyth, \{Malcolm R\} and Mikael Wasberg",

note = "Funding Information: We thank Dr. Tore Lindholm for the field sample, Dr. Kaarina Sivonen for the original Anabaena material, Mrs. Katriina Kurki-Helasmo for growing the cyanobacteria, Mr. Markku Reunanen for the electrospray MS in the characterization of the purified toxins and Mr. Allan Nurmi for technical assistance. B.K. thanks the Erasmus scheme for a travel grant. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1998",

month = jun,

day = "12",

doi = "10.1016/S0021-9673(98)00203-9",

language = "English",

volume = "810",

pages = "226--230",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier",

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TY - JOUR

T1 - Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

AU - Meriluoto, Jussi

AU - Kincaid, Brendan

AU - Smyth, Malcolm R

AU - Wasberg, Mikael

N1 - Funding Information: We thank Dr. Tore Lindholm for the field sample, Dr. Kaarina Sivonen for the original Anabaena material, Mrs. Katriina Kurki-Helasmo for growing the cyanobacteria, Mr. Markku Reunanen for the electrospray MS in the characterization of the purified toxins and Mr. Allan Nurmi for technical assistance. B.K. thanks the Erasmus scheme for a travel grant. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1998/6/12

Y1 - 1998/6/12

N2 - A novel amperometric HPLC detection method for the cyanobacterial (blue- green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins.

AB - A novel amperometric HPLC detection method for the cyanobacterial (blue- green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins.

KW - Cyanobacteria

KW - Detection, LC

KW - Hepatotoxins

KW - Microcystins

KW - Toxins

UR - https://www.scopus.com/pages/publications/0031836852

U2 - 10.1016/S0021-9673(98)00203-9

DO - 10.1016/S0021-9673(98)00203-9

M3 - Article

C2 - 9691298

AN - SCOPUS:0031836852

SN - 0021-9673

VL - 810

SP - 226

EP - 230

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

Abstract

Funding

Keywords

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