Abstract
A novel amperometric HPLC detection method for the cyanobacterial (blue- green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins.
Original language | English |
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Pages (from-to) | 226-230 |
Number of pages | 5 |
Journal | Journal of Chromatography A |
Volume | 810 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 12 Jun 1998 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Cyanobacteria
- Detection, LC
- Hepatotoxins
- Microcystins
- Toxins