Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

Jussi Meriluoto*, Brendan Kincaid, Malcolm R. Smyth, Mikael Wasberg

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

38 Citations (Scopus)

Abstract

A novel amperometric HPLC detection method for the cyanobacterial (blue- green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalJournal of Chromatography A
Volume810
Issue number1-2
DOIs
Publication statusPublished - 12 Jun 1998
MoE publication typeA1 Journal article-refereed

Keywords

  • Cyanobacteria
  • Detection, LC
  • Hepatotoxins
  • Microcystins
  • Toxins

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