Effects of microcystins on broccoli and mustard, and analysis of accumulated toxin by liquid chromatography-mass spectrometry

Suvi Järvenpää; Catharina Lundberg-Niinistö; Lisa Spoof; Olli Sjövall; Esa Tyystjärvi; Jussi Meriluoto

doi:10.1016/j.toxicon.2006.12.008

Effects of microcystins on broccoli and mustard, and analysis of accumulated toxin by liquid chromatography-mass spectrometry

Suvi Järvenpää, Catharina Lundberg-Niinistö, Lisa Spoof, Olli Sjövall, Esa Tyystjärvi, Jussi Meriluoto^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

77 Citations (Scopus)

Abstract

Microcystins (MCs) are cyclic heptapeptides and protein phosphatase inhibitors produced by many species of cyanobacteria. MCs have been shown to cause adverse effects on animals as well as plants and therefore methods are needed for analysing MCs in different matrices. We assessed the effects of MC exposure on broccoli (Brassica oleracea var. italica) and mustard (Sinapis alba) by watering the seedlings with water containing 0, 1 or 10 μg MCs L^-1 (concentrations typically found in natural waters). Morphological characteristics, chlorophyll concentrations and chlorophyll fluorescence were investigated, but the only distinct difference compared to control plants was a slight (<10%) growth inhibition seen in broccoli. Afterwards the MC concentration of selected plant samples was quantitated using liquid chromatography-mass spectrometry. Among the four MC variants present in the exposure mixture, only MC-LR was clearly detectable, and the toxin was found only in the roots of broccoli and mustard. The detected MC-LR concentrations ranged from 0.9 to 2.6 ng (g fresh weight)^-1.

Original language	English
Pages (from-to)	865-874
Number of pages	10
Journal	Toxicon
Volume	49
Issue number	6
DOIs	https://doi.org/10.1016/j.toxicon.2006.12.008
Publication status	Published - May 2007
MoE publication type	A1 Journal article-refereed

Keywords

Accumulation
Cyanobacteria
Microcystin
Plants
Toxin

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10.1016/j.toxicon.2006.12.008

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@article{304fb1ebb48d4ff19994cd75598aeb85,

title = "Effects of microcystins on broccoli and mustard, and analysis of accumulated toxin by liquid chromatography-mass spectrometry",

abstract = "Microcystins (MCs) are cyclic heptapeptides and protein phosphatase inhibitors produced by many species of cyanobacteria. MCs have been shown to cause adverse effects on animals as well as plants and therefore methods are needed for analysing MCs in different matrices. We assessed the effects of MC exposure on broccoli (Brassica oleracea var. italica) and mustard (Sinapis alba) by watering the seedlings with water containing 0, 1 or 10 μg MCs L-1 (concentrations typically found in natural waters). Morphological characteristics, chlorophyll concentrations and chlorophyll fluorescence were investigated, but the only distinct difference compared to control plants was a slight (<10%) growth inhibition seen in broccoli. Afterwards the MC concentration of selected plant samples was quantitated using liquid chromatography-mass spectrometry. Among the four MC variants present in the exposure mixture, only MC-LR was clearly detectable, and the toxin was found only in the roots of broccoli and mustard. The detected MC-LR concentrations ranged from 0.9 to 2.6 ng (g fresh weight)-1.",

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author = "Suvi J{\"a}rvenp{\"a}{\"a} and Catharina Lundberg-Niinist{\"o} and Lisa Spoof and Olli Sj{\"o}vall and Esa Tyystj{\"a}rvi and Jussi Meriluoto",

note = "Funding Information: We thank the Academy of Finland, Research Council for Biosciences and Environment, for financial support (decision numbers 108947 and 110409). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2007",

month = may,

doi = "10.1016/j.toxicon.2006.12.008",

language = "English",

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AU - Järvenpää, Suvi

AU - Lundberg-Niinistö, Catharina

AU - Spoof, Lisa

AU - Sjövall, Olli

AU - Tyystjärvi, Esa

AU - Meriluoto, Jussi

N1 - Funding Information: We thank the Academy of Finland, Research Council for Biosciences and Environment, for financial support (decision numbers 108947 and 110409). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2007/5

Y1 - 2007/5

N2 - Microcystins (MCs) are cyclic heptapeptides and protein phosphatase inhibitors produced by many species of cyanobacteria. MCs have been shown to cause adverse effects on animals as well as plants and therefore methods are needed for analysing MCs in different matrices. We assessed the effects of MC exposure on broccoli (Brassica oleracea var. italica) and mustard (Sinapis alba) by watering the seedlings with water containing 0, 1 or 10 μg MCs L-1 (concentrations typically found in natural waters). Morphological characteristics, chlorophyll concentrations and chlorophyll fluorescence were investigated, but the only distinct difference compared to control plants was a slight (<10%) growth inhibition seen in broccoli. Afterwards the MC concentration of selected plant samples was quantitated using liquid chromatography-mass spectrometry. Among the four MC variants present in the exposure mixture, only MC-LR was clearly detectable, and the toxin was found only in the roots of broccoli and mustard. The detected MC-LR concentrations ranged from 0.9 to 2.6 ng (g fresh weight)-1.

AB - Microcystins (MCs) are cyclic heptapeptides and protein phosphatase inhibitors produced by many species of cyanobacteria. MCs have been shown to cause adverse effects on animals as well as plants and therefore methods are needed for analysing MCs in different matrices. We assessed the effects of MC exposure on broccoli (Brassica oleracea var. italica) and mustard (Sinapis alba) by watering the seedlings with water containing 0, 1 or 10 μg MCs L-1 (concentrations typically found in natural waters). Morphological characteristics, chlorophyll concentrations and chlorophyll fluorescence were investigated, but the only distinct difference compared to control plants was a slight (<10%) growth inhibition seen in broccoli. Afterwards the MC concentration of selected plant samples was quantitated using liquid chromatography-mass spectrometry. Among the four MC variants present in the exposure mixture, only MC-LR was clearly detectable, and the toxin was found only in the roots of broccoli and mustard. The detected MC-LR concentrations ranged from 0.9 to 2.6 ng (g fresh weight)-1.

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Effects of microcystins on broccoli and mustard, and analysis of accumulated toxin by liquid chromatography-mass spectrometry

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Keywords

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