Effects of heat shock and hypoxia on protein synthesis in rainbow trout (Oncorhynchus mykiss) cells

S Airaksinen, CM Råbergh, Lea Sistonen, M Nikinmaa

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    Abstract

    We examined the effects of heat stress (from 18 degrees C to 26 degrees C) and low oxygen tension (1% O-2=1 kPa) on protein synthesis in primary cultures of hepatocytes, gill epithelial cells and fibroblast-like RTG-2 cells of rainbow trout Oncorhynchus mykiss. All these cell types displayed elevated levels of 67, 69 and 92 kDa proteins, whereas a 104 kDa protein was induced only in RTG-2 cells. Hypoxia induced a cell-type-specific response, increasing the synthesis of 36, 39 and 51 kDa proteins in the gill epithelial cells. The regulation of the heat-shock response in fish hepatocytes showed that an HSF1-like factor is involved in the transcriptional induction of the hsp70 gene. Consequently, there was a pronounced accumulation of hsp70 mRNA, Furthermore, the kinetics of activation of DNA binding and the increase in hsp70 gene expression showed a remarkable correlation, indicating that hsp70 expression is regulated at the transcriptional level in these trout cells.
    Original languageUndefined/Unknown
    Pages (from-to)2543–2551
    Number of pages9
    JournalJournal of Experimental Biology
    Volume201
    Issue number17
    Publication statusPublished - 1998
    MoE publication typeA1 Journal article-refereed

    Keywords

    • cellular stress response
    • gene expression
    • heat-shock transcription factor
    • Hsp70
    • Oncorhynchus mykiss
    • pH
    • protein synthesis
    • rainbow trout

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