Effect of caldesmon mutations in the development of zebrafish embryos

Verneri Virtanen; Kreetta Paunu; Saana Niva; Maria Sundvall; Ilkka Paatero

doi:10.1016/j.bbrc.2023.05.079

Effect of caldesmon mutations in the development of zebrafish embryos

Verneri Virtanen
, Kreetta Paunu
, Saana Niva
, Maria Sundvall^*
, Ilkka Paatero^*

^*Corresponding author for this work

Turku Bioscience Centre

Research output: Contribution to journal › Article › Scientific › peer-review

1 Citation (Scopus)

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Abstract

Cancer is a profound medical concern and better treatments are needed for cancer patients. Therefore, new cancer targets are constantly being studied. These targets need not only be relevant for cancer progression, but their modulation needs to be tolerated reasonably well by the host. Caldesmon is one of these proposed novel targets for cancer therapy. Therefore, we analyzed effects of caldesmon mutations in normal development using genetically modified zebrafish embryos. We analyzed mutations in both zebrafish caldesmon genes, cald1a and cald1b and analyzed effects of either mutation alone or as in combination in double homozygous embryos using molecular, morphological and functional analyses. The effects of caldesmon mutations were mild and the gross development of zebrafish embryos was normal. The caldesmon mutant embryos had, however, alterations in response to light-stimulus in behavioural assays. Taken together, the effects of caldesmon mutations in the development of zebrafish embryos were reasonably well tolerated and did not indicate significant concerns for caldesmon being a potential target for cancer therapy.

Original language	English
Pages (from-to)	10-18
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	669
DOIs	https://doi.org/10.1016/j.bbrc.2023.05.079
Publication status	Published - 20 Aug 2023
MoE publication type	A1 Journal article-refereed

Funding

Analyses of zebrafish embryos were carried out under the licenses MMM/465/712–93 (issued by the Finnish Ministry of Agriculture and Forestry) and ESAVI/9339/04.10.07/2016 and ESAVI/31414/2020 (granted by Project Authorization Board of Regional State Administrative Agency for Southern Finland) according to the regulations of the Finnish Act on Animal Experimentation (62/2006). The study was carried out in compliance with the ARRIVE guidelines.This work was supported by grants from Academy of Finland, Finnish Medical Foundation, Finnish Cancer Foundations, Turku University Foundation, Turku University Hospital, TYKS Foundation, Finnish Cultural Foundation, and Turku Doctoral Programme of Molecular Medicine (TuDMM).We thank Zebrafish Core, Cell Imaging Core and Finnish Functional Genomics Centre (all in Turku Bioscience Centre and supported by Biocenter Finland) for services, instrumentation, and consultations. We thank Minna Santanen for excellent technical assistance. This work was supported by grants from Academy of Finland , Finnish Medical Foundation , Finnish Cancer Foundations , Turku University Foundation , Turku University Hospital , TYKS Foundation , Finnish Cultural Foundation , and Turku Doctoral Programme of Molecular Medicine (TuDMM).

Keywords

Caldesmon
Developmental biology
Mutation
Zebrafish

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10.1016/j.bbrc.2023.05.079

1-s2.0-S0006291X23006575-mainFinal published version, 2.63 MBLicence: CC BY

https://urn.fi/URN:NBN:fi-fe202402126605

Cite this

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title = "Effect of caldesmon mutations in the development of zebrafish embryos",

abstract = "Cancer is a profound medical concern and better treatments are needed for cancer patients. Therefore, new cancer targets are constantly being studied. These targets need not only be relevant for cancer progression, but their modulation needs to be tolerated reasonably well by the host. Caldesmon is one of these proposed novel targets for cancer therapy. Therefore, we analyzed effects of caldesmon mutations in normal development using genetically modified zebrafish embryos. We analyzed mutations in both zebrafish caldesmon genes, cald1a and cald1b and analyzed effects of either mutation alone or as in combination in double homozygous embryos using molecular, morphological and functional analyses. The effects of caldesmon mutations were mild and the gross development of zebrafish embryos was normal. The caldesmon mutant embryos had, however, alterations in response to light-stimulus in behavioural assays. Taken together, the effects of caldesmon mutations in the development of zebrafish embryos were reasonably well tolerated and did not indicate significant concerns for caldesmon being a potential target for cancer therapy.",

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AU - Paunu, Kreetta

AU - Niva, Saana

AU - Sundvall, Maria

AU - Paatero, Ilkka

PY - 2023/8/20

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N2 - Cancer is a profound medical concern and better treatments are needed for cancer patients. Therefore, new cancer targets are constantly being studied. These targets need not only be relevant for cancer progression, but their modulation needs to be tolerated reasonably well by the host. Caldesmon is one of these proposed novel targets for cancer therapy. Therefore, we analyzed effects of caldesmon mutations in normal development using genetically modified zebrafish embryos. We analyzed mutations in both zebrafish caldesmon genes, cald1a and cald1b and analyzed effects of either mutation alone or as in combination in double homozygous embryos using molecular, morphological and functional analyses. The effects of caldesmon mutations were mild and the gross development of zebrafish embryos was normal. The caldesmon mutant embryos had, however, alterations in response to light-stimulus in behavioural assays. Taken together, the effects of caldesmon mutations in the development of zebrafish embryos were reasonably well tolerated and did not indicate significant concerns for caldesmon being a potential target for cancer therapy.

AB - Cancer is a profound medical concern and better treatments are needed for cancer patients. Therefore, new cancer targets are constantly being studied. These targets need not only be relevant for cancer progression, but their modulation needs to be tolerated reasonably well by the host. Caldesmon is one of these proposed novel targets for cancer therapy. Therefore, we analyzed effects of caldesmon mutations in normal development using genetically modified zebrafish embryos. We analyzed mutations in both zebrafish caldesmon genes, cald1a and cald1b and analyzed effects of either mutation alone or as in combination in double homozygous embryos using molecular, morphological and functional analyses. The effects of caldesmon mutations were mild and the gross development of zebrafish embryos was normal. The caldesmon mutant embryos had, however, alterations in response to light-stimulus in behavioural assays. Taken together, the effects of caldesmon mutations in the development of zebrafish embryos were reasonably well tolerated and did not indicate significant concerns for caldesmon being a potential target for cancer therapy.

KW - Caldesmon

KW - Developmental biology

KW - Mutation

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KW - Caldesmon

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KW - Zebrafish

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Effect of caldesmon mutations in the development of zebrafish embryos

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