Diatom-inspired skeletonisation of insulin - mechanistic insights into crystallisation and extracellular bioactivity

Diosangeles Soto Véliz; Catharina Alam; Thiago Neitzel; Rebecca Wyborski; Adolfo Rivero-Müller; Parvez Alam

doi:10.1016/j.colsurfb.2015.05.047

Diatom-inspired skeletonisation of insulin - mechanistic insights into crystallisation and extracellular bioactivity

Diosangeles Soto Véliz, Catharina Alam, Thiago Neitzel, Rebecca Wyborski, Adolfo Rivero-Müller, Parvez Alam

Research output: Contribution to journal › Article › Scientific › peer-review

3 Citations (Scopus)

Abstract

In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.

Original language	Undefined/Unknown
Pages (from-to)	140–147
Journal	Colloids and Surfaces B: Biointerfaces
Volume	133
DOIs	https://doi.org/10.1016/j.colsurfb.2015.05.047
Publication status	Published - 2015
MoE publication type	A1 Journal article-refereed

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10.1016/j.colsurfb.2015.05.047

http://www.sciencedirect.com/science/article/pii/S0927776515003604

Cite this

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abstract = "In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.",

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AU - Neitzel, Thiago

AU - Wyborski, Rebecca

AU - Rivero-Müller, Adolfo

AU - Alam, Parvez

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AB - In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.

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