Laccases belong to the family of blue multicopper oxidases, which catalyze the four-electron redn. of dioxygen to water concomitantly through the oxidn. of phenolic and other arom. compds. They are potential enzymes in many applications including biofuel cells to produce electricity through chem. reactions. We have tested here the incorporation of a high redox potential laccase from Trametes hirsuta in different types of conducting inks to produce dry printed enzyme electrode layers. ABTS was used as the redox mediator to shuttle the electrons between the surface of the cathodic electrode and the enzyme active sites. Our results demonstrate that the dry printed layers maintained their enzymic activity even after several months. Furthermore, fuel cell prototypes could be constructed utilizing an optimized printed laccase-ABTS layer as the cathode, and printed Zn layer as the anode. Under humidity controlled conditions, a cell voltage between 0.8 and 0.6 V could be maintained for several days under a 2.2 kω load. In addn., a corresponding stand-alone cell could be constructed where the cell voltage was maintained for 15 h under a load. These results offer a good starting point for further development of mass-producible, completely enzymic printed biofuel cells.