TY - JOUR
T1 - Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry
AU - Neffling, Milla Riina
AU - Lance, Emilie
AU - Meriluoto, Jussi
N1 - Funding Information:
The authors would like to thank the National Graduate school in Informational and Structural Biology, Medicinska understödsföreningen Liv och Hälsa, Otto A. Malms donations fond (M-R N.), and the CIMO (Center for International Mobility) and the French and Finnish Ministries of Foreign Affairs (E.L.), Tekes decision number 40366/06 and Academy of Finland decision number 108947 (J.M.). Dr. Krister Karlsson is thanked for his helpful advice in the early stages of the study.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/3
Y1 - 2010/3
N2 - Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.
AB - Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.
KW - Microcystins
KW - MMPB method
KW - Total contamination levels
UR - http://www.scopus.com/inward/record.url?scp=74149091840&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2009.10.023
DO - 10.1016/j.envpol.2009.10.023
M3 - Article
C2 - 19910094
AN - SCOPUS:74149091840
SN - 0269-7491
VL - 158
SP - 948
EP - 952
JO - Environmental Pollution
JF - Environmental Pollution
IS - 3
ER -