Cyanobacterial hepatotoxins, microcystins and nodularins, in fresh and brackish waters of the Pomeranian Province, northern Poland

Hanna Mazur-Marzec; Lisa Spoof; Justyna Kobos; Marcin Pliński; Jussi Meriluoto

doi:10.2478/v10009-008-0014-0

Cyanobacterial hepatotoxins, microcystins and nodularins, in fresh and brackish waters of the Pomeranian Province, northern Poland

Hanna Mazur-Marzec^*, Lisa Spoof, Justyna Kobos, Marcin Pliński, Jussi Meriluoto

^*Corresponding author for this work

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Abstract

Microcystins (MCs) and structurally related nodularins (NODs) are hepatotoxic cyclic peptides produced by bloom-forming cyanobacteria. These toxins have been implicated in the deaths of wild and domestic animals as well as in incidents of human illness. Cyanobacterial toxins occurring in the fresh and brackish waters of the Pomeranian Province, northern Poland were characterized in this study. Water samples collected from seven lakes in August and September 2005 were analysed by high performance liquid chromatography (HPLC), enzyme linked immunosorbent assay (ELISA) and protein phosphatase inhibition assay (PPIA). Cyanobacterial toxins present in field samples and in an isolated strain of Planktothrix agardhii were also characterized by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In most of the fresh water samples MC-LR, MC-RR and MC-YR dominated. In the lakes where P. agardhii was most abundant demethylated microcystin variants tentatively identified as [D-Asp³]MC-LR, [D-Asp³]MC-YR and [D-Asp³]MC-RR, were found. Total concentrations of the toxins measured by HPLC ranged from 0.1 μg 1^-1 to 305.4 μg 1^-1. Nodularia spumigena bloom samples were collected from brackish waters of the Gulf of Gdańsk, southern Baltic, and LC-ISP-MS/MS of extract from these revealed the presence of two geometrical isomers of linear nodularin and nodularin variant with aspartic acid methyl ester [MeAsp¹(OMe)]NOD.

Original language	English
Pages (from-to)	3-21
Number of pages	19
Journal	Oceanological and Hydrobiological Studies
Volume	37
Issue number	4
DOIs	https://doi.org/10.2478/v10009-008-0014-0
Publication status	Published - 1 Dec 2008
MoE publication type	A1 Journal article-refereed

Funding

We thank Agata Błaszczyk for assistantance with sample collection. This work was financially supported by the Office of the Marshal of the Pomeranian Voivodeship in Poland (grant number 34/UM/DPS/2007). Nodularin mass spectra were obtained on an instrument of the Pomeranian Science and Technology Park in Gdynia. LS and JM acknowledge financial support from the Academy of Finland (RC for Biosciences and Environment), decision number 108947. Dr. Olli Sjövall is thanked for his help with ion trap LC-MS.

Keywords

Cyanobacterial blooms
Cyclic peptides
LC-MS/MS

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10.2478/v10009-008-0014-0

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title = "Cyanobacterial hepatotoxins, microcystins and nodularins, in fresh and brackish waters of the Pomeranian Province, northern Poland",

abstract = "Microcystins (MCs) and structurally related nodularins (NODs) are hepatotoxic cyclic peptides produced by bloom-forming cyanobacteria. These toxins have been implicated in the deaths of wild and domestic animals as well as in incidents of human illness. Cyanobacterial toxins occurring in the fresh and brackish waters of the Pomeranian Province, northern Poland were characterized in this study. Water samples collected from seven lakes in August and September 2005 were analysed by high performance liquid chromatography (HPLC), enzyme linked immunosorbent assay (ELISA) and protein phosphatase inhibition assay (PPIA). Cyanobacterial toxins present in field samples and in an isolated strain of Planktothrix agardhii were also characterized by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In most of the fresh water samples MC-LR, MC-RR and MC-YR dominated. In the lakes where P. agardhii was most abundant demethylated microcystin variants tentatively identified as [D-Asp3]MC-LR, [D-Asp3]MC-YR and [D-Asp3]MC-RR, were found. Total concentrations of the toxins measured by HPLC ranged from 0.1 μg 1-1 to 305.4 μg 1-1. Nodularia spumigena bloom samples were collected from brackish waters of the Gulf of Gda{\'n}sk, southern Baltic, and LC-ISP-MS/MS of extract from these revealed the presence of two geometrical isomers of linear nodularin and nodularin variant with aspartic acid methyl ester [MeAsp1(OMe)]NOD.",

keywords = "Cyanobacterial blooms, Cyclic peptides, LC-MS/MS",

author = "Hanna Mazur-Marzec and Lisa Spoof and Justyna Kobos and Marcin Pli{\'n}ski and Jussi Meriluoto",

note = "Funding Information: We thank Agata B{\l}aszczyk for assistantance with sample collection. This work was financially supported by the Office of the Marshal of the Pomeranian Voivodeship in Poland (grant number 34/UM/DPS/2007). Nodularin mass spectra were obtained on an instrument of the Pomeranian Science and Technology Park in Gdynia. LS and JM acknowledge financial support from the Academy of Finland (RC for Biosciences and Environment), decision number 108947. Dr. Olli Sj{\"o}vall is thanked for his help with ion trap LC-MS. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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doi = "10.2478/v10009-008-0014-0",

language = "English",

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T1 - Cyanobacterial hepatotoxins, microcystins and nodularins, in fresh and brackish waters of the Pomeranian Province, northern Poland

AU - Mazur-Marzec, Hanna

AU - Spoof, Lisa

AU - Kobos, Justyna

AU - Pliński, Marcin

AU - Meriluoto, Jussi

N1 - Funding Information: We thank Agata Błaszczyk for assistantance with sample collection. This work was financially supported by the Office of the Marshal of the Pomeranian Voivodeship in Poland (grant number 34/UM/DPS/2007). Nodularin mass spectra were obtained on an instrument of the Pomeranian Science and Technology Park in Gdynia. LS and JM acknowledge financial support from the Academy of Finland (RC for Biosciences and Environment), decision number 108947. Dr. Olli Sjövall is thanked for his help with ion trap LC-MS. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Microcystins (MCs) and structurally related nodularins (NODs) are hepatotoxic cyclic peptides produced by bloom-forming cyanobacteria. These toxins have been implicated in the deaths of wild and domestic animals as well as in incidents of human illness. Cyanobacterial toxins occurring in the fresh and brackish waters of the Pomeranian Province, northern Poland were characterized in this study. Water samples collected from seven lakes in August and September 2005 were analysed by high performance liquid chromatography (HPLC), enzyme linked immunosorbent assay (ELISA) and protein phosphatase inhibition assay (PPIA). Cyanobacterial toxins present in field samples and in an isolated strain of Planktothrix agardhii were also characterized by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In most of the fresh water samples MC-LR, MC-RR and MC-YR dominated. In the lakes where P. agardhii was most abundant demethylated microcystin variants tentatively identified as [D-Asp3]MC-LR, [D-Asp3]MC-YR and [D-Asp3]MC-RR, were found. Total concentrations of the toxins measured by HPLC ranged from 0.1 μg 1-1 to 305.4 μg 1-1. Nodularia spumigena bloom samples were collected from brackish waters of the Gulf of Gdańsk, southern Baltic, and LC-ISP-MS/MS of extract from these revealed the presence of two geometrical isomers of linear nodularin and nodularin variant with aspartic acid methyl ester [MeAsp1(OMe)]NOD.

AB - Microcystins (MCs) and structurally related nodularins (NODs) are hepatotoxic cyclic peptides produced by bloom-forming cyanobacteria. These toxins have been implicated in the deaths of wild and domestic animals as well as in incidents of human illness. Cyanobacterial toxins occurring in the fresh and brackish waters of the Pomeranian Province, northern Poland were characterized in this study. Water samples collected from seven lakes in August and September 2005 were analysed by high performance liquid chromatography (HPLC), enzyme linked immunosorbent assay (ELISA) and protein phosphatase inhibition assay (PPIA). Cyanobacterial toxins present in field samples and in an isolated strain of Planktothrix agardhii were also characterized by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In most of the fresh water samples MC-LR, MC-RR and MC-YR dominated. In the lakes where P. agardhii was most abundant demethylated microcystin variants tentatively identified as [D-Asp3]MC-LR, [D-Asp3]MC-YR and [D-Asp3]MC-RR, were found. Total concentrations of the toxins measured by HPLC ranged from 0.1 μg 1-1 to 305.4 μg 1-1. Nodularia spumigena bloom samples were collected from brackish waters of the Gulf of Gdańsk, southern Baltic, and LC-ISP-MS/MS of extract from these revealed the presence of two geometrical isomers of linear nodularin and nodularin variant with aspartic acid methyl ester [MeAsp1(OMe)]NOD.

KW - Cyanobacterial blooms

KW - Cyclic peptides

KW - LC-MS/MS

UR - https://www.scopus.com/pages/publications/58149340058

U2 - 10.2478/v10009-008-0014-0

DO - 10.2478/v10009-008-0014-0

M3 - Article

AN - SCOPUS:58149340058

SN - 1730-413X

VL - 37

SP - 3

EP - 21

JO - Oceanological and Hydrobiological Studies

JF - Oceanological and Hydrobiological Studies

IS - 4

ER -

Cyanobacterial hepatotoxins, microcystins and nodularins, in fresh and brackish waters of the Pomeranian Province, northern Poland

Abstract

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Keywords

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