Cholesterol level affects surface charge of lipid membranes in saline solution

Aniket Magarkar; Vivek Dhawan; Paraskevi Kallinteri; Tapani Viitala; Mohammed Elmowafy; Tomasz Róg; Alex Bunker

doi:10.1038/srep05005

Cholesterol level affects surface charge of lipid membranes in saline solution

Aniket Magarkar, Vivek Dhawan, Paraskevi Kallinteri, Tapani Viitala, Mohammed Elmowafy, Tomasz Róg, Alex Bunker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

162 Citations (Scopus)

Abstract

Cholesterol is an important component of all biological membranes as well as drug delivery liposomes. We show here that increasing the level of cholesterol in a phospholipid membrane decreases surface charge in the physiological environment. Through molecular dynamics simulation we have shown that increasing the level of cholesterol decreases Na + ion binding. Complementary experimental ζ - potential measurements have shown a decreased ζ - potential with increasing cholesterol content, indicative of reduced surface charge. Both experiments and simulations have been carried out on both saturated 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and monounsaturated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes. This result is particularly important because membrane surface charge plays an important role in the interactions of biomembranes with peripheral membrane proteins and drug delivery liposomes with the immune system.

Original language	English
Article number	5005
Journal	Scientific Reports
Volume	4
DOIs	https://doi.org/10.1038/srep05005
Publication status	Published - 21 May 2014
Externally published	Yes
MoE publication type	A1 Journal article-refereed

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title = "Cholesterol level affects surface charge of lipid membranes in saline solution",

abstract = "Cholesterol is an important component of all biological membranes as well as drug delivery liposomes. We show here that increasing the level of cholesterol in a phospholipid membrane decreases surface charge in the physiological environment. Through molecular dynamics simulation we have shown that increasing the level of cholesterol decreases Na + ion binding. Complementary experimental ζ - potential measurements have shown a decreased ζ - potential with increasing cholesterol content, indicative of reduced surface charge. Both experiments and simulations have been carried out on both saturated 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and monounsaturated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes. This result is particularly important because membrane surface charge plays an important role in the interactions of biomembranes with peripheral membrane proteins and drug delivery liposomes with the immune system.",

author = "Aniket Magarkar and Vivek Dhawan and Paraskevi Kallinteri and Tapani Viitala and Mohammed Elmowafy and Tomasz R{\'o}g and Alex Bunker",

note = "Funding Information: Authors would like to thank Centre for scientific computing (CSC), Finland for providing computing resources. The authors thank Prof. Arto Urtti for his advice. The Academy of Finland and the Finnish Cultural Foundation funded this work.",

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AU - Magarkar, Aniket

AU - Dhawan, Vivek

AU - Kallinteri, Paraskevi

AU - Viitala, Tapani

AU - Elmowafy, Mohammed

AU - Róg, Tomasz

AU - Bunker, Alex

N1 - Funding Information: Authors would like to thank Centre for scientific computing (CSC), Finland for providing computing resources. The authors thank Prof. Arto Urtti for his advice. The Academy of Finland and the Finnish Cultural Foundation funded this work.

PY - 2014/5/21

Y1 - 2014/5/21

N2 - Cholesterol is an important component of all biological membranes as well as drug delivery liposomes. We show here that increasing the level of cholesterol in a phospholipid membrane decreases surface charge in the physiological environment. Through molecular dynamics simulation we have shown that increasing the level of cholesterol decreases Na + ion binding. Complementary experimental ζ - potential measurements have shown a decreased ζ - potential with increasing cholesterol content, indicative of reduced surface charge. Both experiments and simulations have been carried out on both saturated 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and monounsaturated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes. This result is particularly important because membrane surface charge plays an important role in the interactions of biomembranes with peripheral membrane proteins and drug delivery liposomes with the immune system.

AB - Cholesterol is an important component of all biological membranes as well as drug delivery liposomes. We show here that increasing the level of cholesterol in a phospholipid membrane decreases surface charge in the physiological environment. Through molecular dynamics simulation we have shown that increasing the level of cholesterol decreases Na + ion binding. Complementary experimental ζ - potential measurements have shown a decreased ζ - potential with increasing cholesterol content, indicative of reduced surface charge. Both experiments and simulations have been carried out on both saturated 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and monounsaturated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes. This result is particularly important because membrane surface charge plays an important role in the interactions of biomembranes with peripheral membrane proteins and drug delivery liposomes with the immune system.

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Cholesterol level affects surface charge of lipid membranes in saline solution

Abstract

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