Characterization and diversity of cyano-bacterial hepatotoxins (Microcystins) in blooms from polish freshwaters identified by liquid chromatography-electrospray ionisation mass spectrometry

T. Jurczak*, M. Tarczyñska, K. Karlsson, J. Meriluoto

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

48 Citations (Scopus)

Abstract

Microcystins were determined in 36 cyanobacterial (blue-green algal) bloom samples dominated by Microcystis aeruginosa, Aphanizomenon flos-aquae and Nostoc spp. The analysed samples were collected from 8 water bodies in Poland during summers of 1996-2001. Ten microcystins (MC), three major and seven minor variants, were detected in natural cyanobacterial samples by high-performance liquid chromatography (HPLC). The structures of the microcystins were assigned on the basis of liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS). The main microcystins detected in the blooms from Polish freshwaters were MC-LR, MC-RR and MC-YR. Microcystin content of the blooms varied from a few μg g-1 to 1687 μg g-1 freeze-dried material. Also some monodesmethyl (dm) and didesmethyl (dmdm) variants of MC-LR, MC-RR, MC-YR were found in a few analysed samples. Furthermore, two compounds were tentatively characterised as MC-(H4)YR and dm-MC-FR.

Original languageEnglish
Pages (from-to)571-578
Number of pages8
JournalChromatographia
Volume59
Issue number9
Publication statusPublished - May 2004
MoE publication typeA1 Journal article-refereed

Keywords

  • Column liquid chromatography
  • Cyanobacteria
  • Liquid chromatography-electrospray ionisation mass spectrometry
  • Microcystins
  • Microcystis

Fingerprint

Dive into the research topics of 'Characterization and diversity of cyano-bacterial hepatotoxins (Microcystins) in blooms from polish freshwaters identified by liquid chromatography-electrospray ionisation mass spectrometry'. Together they form a unique fingerprint.

Cite this