Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes

Neeraj Prabhakar, Ilya Belevich, Markus Peurla, Xavier Heiligenstein, Huan-Cheng Chang, Cecilia Sahlgren, Eija Jokitalo, Jessica M Rosenholm

Research output: Contribution to journalArticleScientificpeer-review

1 Downloads (Pure)

Abstract

Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.

Original languageEnglish
Article number14
Number of pages10
JournalNanomaterials
Volume11
Issue number1
DOIs
Publication statusPublished - 23 Dec 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • correlative microscopy
  • 3D CLEM
  • volume imaging
  • nanodiamonds
  • fluorescence

Fingerprint Dive into the research topics of 'Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes'. Together they form a unique fingerprint.

Cite this