Abstract
Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.
Original language | English |
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Article number | 14 |
Number of pages | 10 |
Journal | Nanomaterials |
Volume | 11 |
Issue number | 1 |
DOIs | |
Publication status | Published - 23 Dec 2020 |
MoE publication type | A1 Journal article-refereed |
Keywords
- correlative microscopy
- 3D CLEM
- volume imaging
- nanodiamonds
- fluorescence